Wen Jianchuan, Yeh Chih-Ko, Sun Yuyu
Department of Chemistry, University of Massachusetts Lowell, 1 University Avenue, Lowell, Massachusetts 01854, USA.
J Mater Chem B. 2018 Mar 14;6(10):1452-1457. doi: 10.1039/c7tb02592k. Epub 2018 Feb 23.
Candida-associated denture stomatitis (CADS) is a common, recurring clinical complication in denture wearers that can lead to serious oral and systemic health problems. Current management strategies are not satisfactory due to their short-acting and ineffective therapeutic effects. Here, we describe a new fungal biofilm controlling strategy using the polyelectrolyte layer-by-layer (LBL) self-assembly technology on denture materials. Conventional poly(methyl methacrylate) (PMMA) denture material discs were functionalized with negatively charged poly(methacrylic acid) (PMAA) via plasma-initiated surface grafting, followed by repetitive alternating coating with the salivary antimicrobial polypeptide histatin 5 (H-5; cationic polymer) and hyaluronic acid (HA; anionic polymer). On the other hand, the H-5/HA LBL coatings (i.e., the outermost layer was H-5) inhibited fungal attachment/adhesion, significantly reduced fungal biofilm formation, and showed synergistic effects with the antifungal drug miconazole. LBL surface hydrophilicity was not the key mechanism in controlling Candida biofilm formation. The current approach demonstrates the utility of a new design principle for fabricating anticandidal denture materials, as well as potentially other related medical devices, for controlling fungal biofilm formation and combating insidious infections.
念珠菌相关性义齿性口炎（CADS）是义齿佩戴者常见的复发性临床并发症，可导致严重的口腔和全身健康问题。由于目前的治疗策略作用时间短且治疗效果不佳，因此并不令人满意。在此，我们描述了一种新的真菌生物膜控制策略，即在义齿材料上使用聚电解质层层（LBL）自组装技术。通过等离子体引发的表面接枝，用带负电荷的聚甲基丙烯酸（PMAA）对传统的聚甲基丙烯酸甲酯（PMMA）义齿材料圆盘进行功能化处理，然后用唾液抗菌多肽组蛋白5（H-5；阳离子聚合物）和透明质酸（HA；阴离子聚合物）进行重复交替涂层。另一方面，H-5/HA LBL涂层（即最外层为H-5）抑制真菌附着/黏附，显著减少真菌生物膜形成，并与抗真菌药物咪康唑显示出协同作用。LBL表面亲水性不是控制念珠菌生物膜形成的关键机制。目前的方法证明了一种新的设计原则在制造抗念珠菌义齿材料以及潜在的其他相关医疗设备方面的实用性，用于控制真菌生物膜形成和对抗隐匿性感染。
