Monaco Ilaria, Armanetti Paolo, Locatelli Erica, Flori Alessandra, Maturi Mirko, Del Turco Serena, Menichetti Luca, Comes Franchini Mauro
Department of Industrial Chemistry "Toso Montanari", University of Bologna, Viale Risorgimento 4, Bologna 40136, Italy.
J Mater Chem B. 2018 May 21;6(19):2993-2999. doi: 10.1039/c8tb00453f. Epub 2018 Apr 25.
Herein we report the synthesis of a resilient nanosystem based on silica-coated magnetic MnFeO nanoparticles conjugated to fluorescein and PEGylated gold nanorods embedded in polymeric micelles (MnFeO@SiO@GNRs@PMs), for magnetic-photoacoustic-optical triple-modality imaging. The magnetic relaxivity of the nanosystem has been evaluated, revealing high r/r ratios that suggest the effectiveness of the nanosystem as the T-contrast agent. In addition, contrast-based fluorescence imaging has been tested both in vitro and ex vivo, showing that the nanosystem exhibits the suitable optical properties of fluorescein, with contrast intensities comparable with previously reported results. Finally, photoacoustic, due to gold nanorods, performances of the nanosystem have been evaluated, revealing good linearity between concentration and photoacoustic response in the 25-250 nM concentration under irradiation at 690 nm. The results showed a contrast-to-noise ratio (CNR) as high as 60 in a mouse leg subcutaneously injected with the nanosystem. Biocompatibility studies revealed no hemolytic effect induced by the nanoconstruct, revealing the applicability of the studied diagnostic tool for medical studies.
在此，我们报告了一种基于二氧化硅包覆的磁性MnFeO纳米颗粒与荧光素共轭以及聚乙二醇化金纳米棒嵌入聚合物胶束（MnFeO@SiO@GNRs@PMs）的弹性纳米系统的合成，用于磁光声三模态成像。已评估了该纳米系统的磁弛豫率，结果显示高的r/r比，表明该纳米系统作为T2造影剂的有效性。此外，已在体外和离体条件下测试了基于对比度的荧光成像，结果表明该纳米系统具有荧光素合适的光学性质，其对比度强度与先前报道的结果相当。最后，由于金纳米棒，已评估了该纳米系统的光声性能，结果显示在690nm照射下，在25 - 250 nM浓度范围内，浓度与光声响应之间具有良好的线性关系。结果表明，在皮下注射该纳米系统的小鼠腿部，对比度噪声比（CNR）高达60。生物相容性研究表明，该纳米结构未诱导溶血效应，表明所研究的诊断工具适用于医学研究。
