Department of Plastic and Reconstructive Surgery, Faculty of Medicine, "Victor Babes" University of Medicine and Pharmacy of Timisoara, 300041 Timisoara, Romania.
Department of Clinical Practical Skills, Faculty of Medicine, "Victor Babes" University of Medicine and Pharmacy of Timisoara, 300041 Timisoara, Romania.
Int J Mol Sci. 2024 Jul 31;25(15):8380. doi: 10.3390/ijms25158380.
In vitro alternative therapy of human epidermoid squamous carcinoma (A431) by superparamagnetic hyperthermia (SPMHT) using FeO (magnetite) superparamagnetic nanoparticles (SPIONs) with an average diameter of 15.8 nm, bioconjugated with hydroxypropyl gamma-cyclodextrins (HP-γ-CDs) by means of polyacrylic acid (PAA) biopolymer, is presented in this paper. The therapy was carried out at a temperature of 43 °C for 30 min using the concentrations of FeO ferrimagnetic nanoparticles from nanobioconjugates of 1, 5, and 10 mg/mL nanoparticles in cell suspension, which were previously found by us to be non-toxic for healthy cells (cell viabilities close to 100%), according to ISO standards (cell viability must be greater than 70%). The temperature for the in vitro therapy was obtained by the safe application (without exceeding the biological limit and cellular damage) of an alternating magnetic field with a frequency of 312.4 kHz and amplitudes of 168, 208, and 370 G, depending on the concentration of the magnetic nanoparticles. The optimal concentration of magnetic nanoparticles in suspension was found experimentally. The results obtained after the treatment show its high effectiveness in destroying the A431 tumor cells, up to 83%, with the possibility of increasing even more, which demonstrates the viability of the SPMHT method with FeO-PAA-(HP-γ-CDs) nanobioconjugates for human squamous cancer therapy.
本文介绍了使用平均直径为 15.8nm 的 FeO（磁铁矿）超顺磁纳米粒子（SPION）通过聚丙烯酸（PAA）生物聚合物与羟丙基γ-环糊精（HP-γ-CDs）生物结合，对人类表皮鳞状癌细胞（A431）进行体外超顺磁热疗（SPMHT）的替代疗法。在 43°C 的温度下，通过浓度为 1、5 和 10mg/mL 的纳米生物复合物中的 FeO 铁磁纳米粒子的细胞悬浮液进行了 30 分钟的治疗，根据 ISO 标准（细胞活力必须大于 70%），我们之前发现这些浓度对健康细胞没有毒性（细胞活力接近 100%）。体外治疗的温度是通过应用安全（不超过生物限制和细胞损伤）的频率为 312.4 kHz 的交变磁场获得的，磁场的幅度为 168、208 和 370 G，具体取决于磁性纳米粒子的浓度。通过实验找到了悬浮液中磁性纳米粒子的最佳浓度。治疗后的结果表明，该方法在破坏 A431 肿瘤细胞方面非常有效，高达 83%，甚至可能更高，这证明了使用 FeO-PAA-(HP-γ-CDs)纳米生物复合物进行 SPMHT 治疗人类鳞状癌的方法具有可行性。
