Deng Yanglong, Liu Minjun, Chen Xuening, Wang Menglu, Li Xiangfeng, Xiao Yumei, Zhang Xingdong
National Engineering Research Center for Biomaterials, Sichuan University, Chengdu 610064, China.
J Mater Chem B. 2018 Nov 7;6(41):6572-6584. doi: 10.1039/c8tb01637b. Epub 2018 Sep 28.
Further biomimicking natural bone and enhancing osteoinductivity to meet the requirements of regenerative medicine is the key development direction of biphasic calcium phosphate (BCP) ceramics. Bone mineral is a kind of Ca-deficient hydroxyapatite (CDHA) with many kinds of trace ions incorporated; however, little is actually known regarding the incorporation of trace ions in CDHA, and trace-ions-incorporated CDHA in BCP ceramics has seldom been studied. The present study introduces an effective approach to fabricate porous BCP ceramic beads with a high content of strontium (Sr)-incorporated CDHA (BCP-Sr), and investigated its biological performance, especially with regard to osteoinductivity. The obtained BCP-Sr possessed a good spherical shape, interconnected pore structure, and a high content of Sr-incorporated CDHA phase. Compared to the commercial BCP ceramic irregular granules (BAM® P2040, BCP-C), BCP-Sr had more micropores, relatively faster degradation, better bone-like apatite formation, higher protein adsorption abilities, and was more likely to promote the related osteogenic genes and protein expressions of BMSCs. Further in vivo canine intramuscular implantation confirmed that BCP-Sr had higher osteoinductivity than BCP-C. Collectively, the enhanced osteoinductivity of porous BCP ceramic beads by introducing a high content of Sr-incorporated CDHA has significant implications for designing highly bioactive BCP ceramics for applying in regenerative medicine.
进一步模拟天然骨并增强骨诱导性以满足再生医学的需求是双相磷酸钙（BCP）陶瓷的关键发展方向。骨矿物质是一种含有多种微量元素的缺钙羟基磷灰石（CDHA）；然而，关于微量元素在CDHA中的掺入实际了解甚少，并且很少有人研究BCP陶瓷中掺入微量元素的CDHA。本研究介绍了一种制备高含量掺锶（Sr）CDHA（BCP-Sr）的多孔BCP陶瓷珠的有效方法，并研究了其生物学性能，特别是骨诱导性。所获得的BCP-Sr具有良好的球形、相互连通的孔隙结构以及高含量的掺Sr CDHA相。与市售的BCP陶瓷不规则颗粒（BAM® P2040，BCP-C）相比，BCP-Sr具有更多的微孔、相对更快的降解、更好的类骨磷灰石形成、更高的蛋白质吸附能力，并且更有可能促进骨髓间充质干细胞（BMSCs）相关成骨基因和蛋白质的表达。进一步的体内犬肌肉植入证实，BCP-Sr比BCP-C具有更高的骨诱导性。总的来说，通过引入高含量的掺Sr CDHA来增强多孔BCP陶瓷珠的骨诱导性对于设计用于再生医学的高生物活性BCP陶瓷具有重要意义。
