Zhao Qi, Huo Fangjun, Kang Jin, Zhang Yongbin, Yin Caixia
Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Key Laboratory of Materials for Energy Conversion and Storage of Shanxi Province, Institute of Molecular Science, Shanxi University, Taiyuan 030006, China.
J Mater Chem B. 2018 Aug 14;6(30):4903-4908. doi: 10.1039/c8tb01070f. Epub 2018 Jul 20.
Hydrogen sulfide (HS) is an endogenously produced gaseous signaling molecule with multiple functions in many physiological and pathological processes. Herein, our group developed a novel fluorescent probe (Flu-N) for HS on the basis of the 7-amino-4-methylcoumarin and fluorescein FRET system. Adding HS to a solution of Flu-N resulted in a clearly observable fluorescence enhancement (50-fold). This probe showed high sensitivity and selectivity towards HS over other reactive species with a detection limit of 0.031 μM and a fast response time (within 8 s). Moreover, Flu-N displayed little toxicity to HepG-2 cells and was successfully applied to image exogenous and endogenous HS in living cells and nude mice.
硫化氢（HS）是一种内源性产生的气态信号分子，在许多生理和病理过程中具有多种功能。在此，我们团队基于7-氨基-4-甲基香豆素和荧光素荧光共振能量转移（FRET）系统开发了一种新型的HS荧光探针（Flu-N）。向Flu-N溶液中加入HS会导致明显可观察到的荧光增强（50倍）。该探针对HS表现出高灵敏度和选择性，优于其他活性物质，检测限为0.031 μM，响应时间快（8秒内）。此外，Flu-N对HepG-2细胞几乎没有毒性，并成功应用于活细胞和裸鼠中外源和内源HS的成像。
