Alam Said, Tao Xuanzuo, Mao Yanxia, Zheng Shaojun, Jiang Chunhui, Chen Shu-Yang, Lu Hongfei
School of Environmental and Chemical Engineering, Jiangsu University of Science and Technology, Zhenjiang, 212000, China.
College of Chemistry and Pharmaceutical Engineering, Nanyang Normal University, Nanyang, 473061, China.
Photochem Photobiol Sci. 2025 Mar;24(3):405-415. doi: 10.1007/s43630-025-00695-x. Epub 2025 Mar 1.
Hydrogen sulfide (HS) is a significant reactive sulfur species (RSS) involved in various human diseases, also playing an important role in many physiological and pathological processes. Thus, the development of an effective method for detecting HS in mammalian cells is of great importance. In this study, we present the synthesis of a novel pyrene-based fluorescent probe, DPP, specifically designed for the selective detection of HS. The DPP exhibits remarkable sensitivity, with a low detection limit of 0.63 µM, and demonstrates high selectivity for HS in the presence of various interfering species. Additionally, the probe has demonstrated rapid detection of HS in less than 6 min. The detection mechanism was thoroughly validated using H NMR, FT-IR, UV and fluorescence spectra. Moreover, the applicability of DPP was successfully demonstrated in both in vitro and in vivo settings using HeLa cells, confirming its potential as a powerful tool for monitoring HS in biological systems. Additionally, the probe exhibited excellent performance in detecting HS in water samples and in paper strip-based assays, further highlighting its versatility and practical utility for environmental monitoring and on-site applications.
硫化氢（HS）是一种重要的反应性硫物种（RSS），与多种人类疾病相关，在许多生理和病理过程中也发挥着重要作用。因此，开发一种在哺乳动物细胞中检测HS的有效方法至关重要。在本研究中，我们展示了一种新型芘基荧光探针DPP的合成，该探针专为选择性检测HS而设计。DPP表现出显著的灵敏度，检测限低至0.63 µM，并且在存在各种干扰物种的情况下对HS具有高选择性。此外，该探针已证明在不到6分钟内即可快速检测HS。使用核磁共振氢谱（H NMR）、傅里叶变换红外光谱（FT-IR）、紫外光谱和荧光光谱对检测机制进行了充分验证。此外，使用HeLa细胞在体外和体内环境中成功证明了DPP的适用性，证实了其作为监测生物系统中HS的有力工具的潜力。此外，该探针在检测水样和基于纸条的检测中表现出优异的性能，进一步突出了其在环境监测和现场应用中的多功能性和实际实用性。
