Univ. Lille, CNRS, Centrale Lille, ISEN, Univ. Valenciennes, UMR 8520 - IEMN, F-59000 Lille, France.
J Mater Chem B. 2019 May 7;7(17):2771-2781. doi: 10.1039/c8tb01676c. Epub 2019 Apr 9.
Bacterial infections are one of the leading causes of disease worldwide. Conventional antibiotics are becoming less efficient, due to antibiotic-resistant bacterial strains. Therefore, the development of novel antibacterial materials and advanced treatment strategies are becoming increasingly important. In the present work, we developed a simple and efficient strategy for effective bacterial capture and their subsequent eradication through photothermal killing. The developed device consists of a flexible nanoheater, comprising a Kapton/Au nanoholes substrate, coated with reduced graphene oxide-polyethyleneimine (K/Au NH/rGO-PEI) thin films. The Au NH plasmonic structure was tailored to feature strong absorption in the near-infrared (NIR) region, where most biological matter has limited absorption, while PEI was investigated for its strong binding with bacteria through electrostatic interactions. The K/Au NH/rGO-PEI device was demonstrated to capture and eliminate effectively both planktonic Gram-positive Staphilococcus aureus (S. aureus) and Gram-negative Escherichia coli (E. coli) bacteria after 10 min of NIR (980 nm) irradiation and, to destroy and eradicate Staphilococcus epidermidis (S. epidermidis) biofilms after 30 min irradiation. The technique developed herein is simple and universal with potential applications for eradication of different micro-organisms.
细菌感染是全球疾病的主要原因之一。由于抗生素耐药性细菌株的出现，传统抗生素的效果变得越来越差。因此，开发新型抗菌材料和先进的治疗策略变得越来越重要。在本工作中，我们开发了一种简单而有效的策略，通过光热杀伤来有效捕获细菌并随后将其消灭。所开发的装置由柔性纳米加热器组成，该加热器由涂有还原氧化石墨烯-聚乙烯亚胺（rGO-PEI）薄膜的聚酰亚胺/金纳米孔（Kapton/Au NH）基底组成。Au NH 等离子体结构被调整为在近红外（NIR）区域具有强吸收，而大多数生物物质在该区域的吸收有限，而 PEI 则通过静电相互作用被研究用于与细菌的强结合。该 K/Au NH/rGO-PEI 装置在 10 分钟的近红外（980nm）照射后，能有效捕获并消灭浮游革兰氏阳性金黄色葡萄球菌（S. aureus）和革兰氏阴性大肠杆菌（E. coli）细菌，并在 30 分钟照射后能破坏和消灭表皮葡萄球菌（S. epidermidis）生物膜。本研究开发的技术简单通用，具有消灭不同微生物的潜在应用。
