School of Pharmacy, The University of Jordan, Amman 11942, Jordan.
Faculty of Pharmacy, Al-Zaytoonah University of Jordan, Amman 11733, Jordan.
Molecules. 2019 Jul 23;24(14):2661. doi: 10.3390/molecules24142661.
In this study, the photothermal-induced bactericidal activity of phospholipid-decorated gold nanorods (DSPE-AuNR) suspension against planktonic and biofilm cultures was investigated. We found that the treatment of planktonic culture of with DSPE-AuNR suspension (0.25-0.03 nM) followed by a continuous laser beam exposure resulted in ~6 log cycle reduction of the bacterial viable count in comparison to the control. The percentage reduction of biofilm viable count was ~2.5-6.0 log cycle upon laser excitation with different concentrations of DSPE-AuNR as compared to the control. The photothermal ablation activity of DSPE-AuNR (0.125 nM) loaded into poloxamer 407 hydrogel against biofilm resulted in ~4.5-5 log cycle reduction in the biofilm viable count compared to the control. Moreover, transmission electron microscope (TEM) images of the photothermally-treated bacteria revealed a significant change in the bacterial shape and lysis of the bacterial cell membrane in comparison to the untreated bacteria. Furthermore, the results revealed that continuous and pulse laser beam modes effected a comparable photothermal-induced bactericidal activity. Therefore, it can be concluded that phospholipid-coated gold nanorods present a promising nanoplatform to eradicate biofilm responsible for common skin diseases.
在这项研究中，研究了磷脂修饰的金纳米棒（DSPE-AuNR）悬浮液对浮游和生物膜培养物的光热诱导杀菌活性。我们发现，用 DSPE-AuNR 悬浮液（0.25-0.03 nM）处理浮游培养物，然后连续激光照射，与对照组相比，细菌活菌数减少了约 6 个对数周期。与对照组相比，用不同浓度的 DSPE-AuNR 激光激发时，生物膜活菌数减少了约 2.5-6.0 个对数周期。与对照组相比，装载到泊洛沙姆 407 水凝胶中的 DSPE-AuNR（0.125 nM）对生物膜的光热消融活性导致生物膜活菌数减少了约 4.5-5 个对数周期。此外，与未经处理的细菌相比，光热处理后的细菌的透射电子显微镜（TEM）图像显示出细菌形状的显著变化和细菌细胞膜的裂解。此外，结果表明，连续和脉冲激光束模式对光热诱导的杀菌活性有类似的影响。因此，可以得出结论，磷脂包覆的金纳米棒是一种很有前途的纳米平台，可以消除引起常见皮肤病的生物膜。
