Andreeva Alfia, Nikolaeva Oksana, Altynbekov Oleg, Galieva Chulpan, Ilina Kseniia
Department of Infectious Diseases, Zoohygiene and Veterinary Sanitary Inspection of Federal State Budget Educational Institution of Higher Education "Bashkir State Agrarian University", Ufa 450001, Republic Bashkortostan, Russian Federation.
Vet World. 2020 Feb;13(2):238-244. doi: 10.14202/vetworld.2020.238-244. Epub 2020 Feb 10.
The research aimed to study the effect of interferon (IFN)-based drugs on the behavior of immunological parameters in calves during the specific prevention of associative infections.
The object of research was 45 black motley cows and their calves from birth to 2 months of life. Serum and colostrum samples were screened for antibodies against , diarrhea, and coronavirus using serological methods. The testing was performed before vaccination, 40 days before calving, 20 days before calving, and before calving. Colostrum samples were taken during the first milk yield. Serum samples from calves were drawn before colostrum feeding as well as at 7, 14, and 21 days, and 1 and 2 months of age. To measure the level of immunoglobulins A, M, and G, additional serum samples were collected from calves at 25, 35, 65, and 75 days after birth.
Giving pregnant cows, an IFN-based drug at a dose of 1 ml/kg 48 h before vaccination results in the development and accumulation of antibodies to , coronavirus, and viral diarrhea (VD) in the colostrum, with a titer of 7.6±0.3 log, 5.8±0.34 log, and 4.4±0.18 log, respectively. It indicates an increase in the antigenic activity of the multivalent vaccine.
IFN-based drugs enhance the protective effect of vaccination against associative infections in the newborn calves. They stimulate a rise in the titer of antibodies to , coronavirus, VD, and mucosal disease complex as well as an increase in immunoglobulins A, M, and G.
本研究旨在探讨基于干扰素（IFN）的药物在犊牛特异性预防相关感染期间对免疫参数行为的影响。
研究对象为45头黑色花斑奶牛及其出生至2月龄的犊牛。采用血清学方法对血清和初乳样本进行抗体筛查，检测针对[具体病原体未提及]、腹泻和冠状病毒的抗体。检测在疫苗接种前、产犊前40天、产犊前20天以及产犊前进行。初乳样本在首次产奶时采集。犊牛的血清样本在初乳喂养前以及7、14和21天、1月龄和2月龄时采集。为测量免疫球蛋白A、M和G的水平，在犊牛出生后25、35、65和75天采集额外的血清样本。
在疫苗接种前48小时给怀孕母牛注射剂量为1 ml/kg的基于IFN的药物，可导致初乳中针对[具体病原体未提及]、冠状病毒和病毒性腹泻（VD）的抗体产生和积累，滴度分别为7.6±0.3 log、5.8±0.34 log和4.4±0.18 log。这表明多价疫苗的抗原活性增加。
基于IFN的药物可增强新生犊牛针对相关感染的疫苗接种保护效果。它们刺激针对[具体病原体未提及]、冠状病毒、VD和黏膜病复合体的抗体滴度升高，以及免疫球蛋白A、M和G增加。
