Cheng Qun, Gan Zhuoran, Wang Yanping, Lu Sijia, Hou Zhihong, Li Haiyang, Xiang Hongtao, Liu Baohui, Kong Fanjiang, Dong Lidong
Innovative Center of Molecular Genetics and Evolution, School of Life Sciences, Guangzhou University, Guangzhou, China.
Heilongjiang Academy of Agricultural Sciences, Mudanjiang, China.
Front Plant Sci. 2020 Mar 17;11:272. doi: 10.3389/fpls.2020.00272. eCollection 2020.
Soybean [ (L.) Merr.] is an important crop for oil and protein resources worldwide, and its farming is impacted by increasing soil salinity levels. In the gene (), increased salt tolerance by suppressing salt stress response pathways. is the ortholog of in soybean, and loss-of-function -alleles greatly prolong soybean maturity and enhance grain yield. The exact role of in abiotic stress response in soybean, however, remains unclear. In this study, we showed that expression was induced by NaCl treatment and that the J protein was located in the nucleus. Compared to NIL-, tolerance to NaCl was significantly lower in the NIL- mutant. We also demonstrated that overexpression of increased NaCl tolerance in transgenic soybean hairy roots. J positively regulated expression of downstream salt stress response genes, including , , , and . Our study disclosed a mechanism in soybean for regulation of the salt stress response. Manipulation of these genes should facilitate improvements in salt tolerance in soybean.
大豆[（L.）Merr.]是全球重要的油脂和蛋白质资源作物，其种植受到土壤盐度上升的影响。在基因（）中，通过抑制盐胁迫反应途径提高了耐盐性。是大豆中 的直系同源基因，功能缺失的等位基因极大地延长了大豆的成熟期并提高了籽粒产量。然而，在大豆非生物胁迫反应中的确切作用仍不清楚。在本研究中，我们发现NaCl处理可诱导表达，且J蛋白定位于细胞核。与NIL-相比，NIL-突变体对NaCl的耐受性显著降低。我们还证明，在转基因大豆毛状根中过表达可提高对NaCl的耐受性。J正向调节下游盐胁迫反应基因的表达，包括 、 、 和 。我们的研究揭示了大豆中盐胁迫反应的调控机制。对这些基因的操作应有助于提高大豆的耐盐性。
