Chioccarelli Teresa, Manfrevola Francesco, Migliaccio Marina, Altucci Lucia, Porreca Veronica, Fasano Silvia, Cobellis Gilda
Department of Experimental Medicine, Sez. Bottazzi, Università degli Studi della Campania "L. Vanvitelli", Via Costantinopoli 16, 80138 Napoli, Italy.
Department of Precision Medicine, Università degli Studi della Campania "L. Vanvitelli", Via L. De Crecchio 7, 80138 Napoli, Italy.
Int J Endocrinol. 2020 Mar 20;2020:2750501. doi: 10.1155/2020/2750501. eCollection 2020.
Bisphenol-A (BPA) is considered an endocrine disruptor with estrogenic activity. It is described as an environment-polluting industrial chemical whose adverse effects on the male reproductive system depend on the period of exposure (i.e., fetal, prepubertal, or adult life). We exposed male mice to BPA during the fetal-perinatal period (from 10 days post to 31 days post ) and investigated the impact of this early-life exposure on gamete health in adulthood animals at 78 days of age. Both in control and BPA-exposed mice, viability and motility of spermatozoa, as well as sperm motility acquisition and chromatin condensation of spermatozoa, have been evaluated. Results reveal harmful effect of BPA on viability and motility of sperm cells as well as on chromatin condensation status during epididymal maturation of spermatozoa. In particular, BPA exposure interferes with biochemical mechanism useful to stabilize sperm chromatin condensation, as it interferes with oxidation of thiol groups associated to chromatin.
双酚A（BPA）被认为是一种具有雌激素活性的内分泌干扰物。它被描述为一种污染环境的工业化学品，其对雄性生殖系统的不利影响取决于暴露时期（即胎儿期、青春期前或成年期）。我们在胎儿围产期（从出生后10天到出生后31天）将雄性小鼠暴露于双酚A，并研究这种早期暴露对78日龄成年动物配子健康的影响。在对照小鼠和暴露于双酚A的小鼠中，均评估了精子的活力和运动能力，以及精子运动能力的获得和精子染色质浓缩情况。结果显示，双酚A对精子细胞的活力和运动能力以及精子在附睾成熟过程中的染色质浓缩状态具有有害影响。特别是，双酚A暴露会干扰有助于稳定精子染色质浓缩的生化机制，因为它会干扰与染色质相关的巯基的氧化。
