Division of Molecular Epidemiology and Public Health, ICMR-Regional Medical Research Centre, Bhubaneswar, Odisha, India.
Division of Vector Borne Diseases, ICMR-National Institute of Research in Tribal Health, Jabalpur, India.
Biomed Res Int. 2020 Mar 15;2020:8475246. doi: 10.1155/2020/8475246. eCollection 2020.
Estimation of the spread and advancement of artemisinin-resistant parasites can be done by probing polymorphisms in the kelch () domain (a validated molecular marker). This study aimed to provide baseline information for future artemisinin surveillance by analyzing the -propeller domain in field isolates collected from 24 study areas in 14 malaria hot spots of Odisha (previously Orissa) during July 2018-January 2019. A total of 178 mono infections were assessed. An 849-base pair fragment encoding the propeller was amplified by nested polymerase chain reaction and sequenced in both directions (PCR). After DNA alignment with the 3D7 reference sequence, all samples were found to be wild type. It can be anticipated that malaria public health is not under direct threat in Odisha relating to ART resistance.
可以通过探测kelch()结构域（一个经过验证的分子标记）中的多态性来估计青蒿素耐药寄生虫的传播和进展。本研究旨在通过分析 2018 年 7 月至 2019 年 1 月在奥里萨邦（以前的奥里萨邦）14 个疟疾热点的 24 个研究地区采集的现场分离株中的-推进器结构域，为未来的青蒿素监测提供基线信息。总共评估了 178 例单感染。通过巢式聚合酶链反应（PCR）扩增编码 推进器的 849 个碱基对片段，并进行双向测序。与 3D7 参考序列进行 DNA 比对后，所有样本均为野生型。可以预期，奥里萨邦的疟疾公共卫生不会直接受到与 ART 耐药性相关的威胁。
