Cakir Sevki, Gultekin B Alper, Karabagli Murat, Yilmaz Tugba Ekiz, Cakir Esra, Guzel E Elif, Yalcin Serdar, Mortellaro Carmen, Mijiritsky Eitan
Department of Oral Implantology, Istanbul University Faculty of Dentistry.
Department of Surgery, Istanbul University Faculty of Veterinary Medicine.
J Craniofac Surg. 2019 Jun;30(4):1078-1084. doi: 10.1097/SCS.0000000000005339.
The aim of this study was to evaluate the effect of mineralized plasmatic matrix (MPM), comprising a combination of synthetic graft and platelet concentrates, on bone regeneration.
Critical size defects of 6-mm diameter were created on the tibias of 6 male sheep, with the animals subsequently assigned into 2 groups. Of the 5 bone defects generated per animal, 4 were randomly filled with MPM, beta-tricalcium phosphate graft (β-TCP), platelet-rich fibrin (PRF) + β-TCP, and autogenous graft. One defect was left empty as a control group. Animals were killed at 3 weeks (early healing group) and 6 weeks (late healing group). The specimens underwent histologic and histomorphometric analysis to evaluate new bone formation.
In both healing periods, new bone formation from autogenous bone was observed significantly more often than from biomaterials or the empty defect. The degree of new bone formation for MPM was significantly higher than that of the control group at all healing periods. In addition, it was significantly higher in both healing periods than that of β-TCP albeit only in the late healing period than that of the PRF + β-TCP combination. In all biomaterial groups, residual graft ratios decreased from early to late healing periods.
The results indicated that MPM, representing growth factors in a fibrin network, increases new bone formation in surgically created defects in sheep tibia as confirmed by histologic assessment.
本研究旨在评估由合成移植物和浓缩血小板组合而成的矿化血浆基质（MPM）对骨再生的影响。
在6只雄性绵羊的胫骨上制造直径为6毫米的临界尺寸骨缺损，随后将动物分为2组。每只动物产生的5个骨缺损中，4个随机填充MPM、β-磷酸三钙移植物（β-TCP）、富血小板纤维蛋白（PRF）+β-TCP和自体移植物。一个缺损留空作为对照组。在3周（早期愈合组）和6周（晚期愈合组）处死动物。对标本进行组织学和组织形态计量学分析以评估新骨形成。
在两个愈合期，自体骨的新骨形成明显比生物材料或空缺损更常见。在所有愈合期，MPM的新骨形成程度均显著高于对照组。此外，在两个愈合期，其新骨形成程度均显著高于β-TCP，尽管仅在晚期愈合期高于PRF+β-TCP组合。在所有生物材料组中，从早期到晚期愈合期，残余移植物比例均下降。
结果表明，如组织学评估所证实，代表纤维蛋白网络中生长因子的MPM可增加绵羊胫骨手术造成的缺损中的新骨形成。
