Chen Jing, Ge Juan, Guo Baolin, Gao Kun, Ma Peter X
Frontier Institute of Science and Technology, and State Key Laboratory for Mechanical Behavior of Materials, Xi'an Jiaotong University, Xi'an, 710049, China.
J Mater Chem B. 2016 Apr 14;4(14):2477-2485. doi: 10.1039/c5tb02703a. Epub 2016 Mar 22.
Nanofibrous electroactive composite scaffolds with sustained release properties based on polylactide (PLA) nanofibers and electroactive nanoparticles from polyurethane-urea copolymers were fabricated for tissue engineering applications. Electroactive polyurethane-urea copolymers were fabricated into nanoparticles which possessed sustained release kinetics for both hydrophobic and hydrophilic drugs. Composite scaffolds were obtained using an electrospray/electrospinning procedure with electroactive polyurethane-urea nanoparticles universally coated on PLA nanofibers. The morphology of nanoparticles, PLA fibers, and composite scaffolds was observed by SEM. The drug release profile of composite scaffolds was studied using ibuprofen and rutin as model drugs. The mechanism of the sustained release of the drugs was investigated. Rutin loaded composite material was used as an active scaffold for L929 fibroblast proliferation. Both random and aligned structures of electroactive composite PLA nanofibrous scaffolds were fabricated. The effect of surface chemistry and topographical cues on C2C12 cell proliferation and differentiation was studied on composite scaffolds, and it was found that electroactivity and aligned morphology of the composite nanofibers provide a synergetic effect for C2C12 myoblast proliferation and differentiation. These electroactive nanofibrous composite scaffolds with sustained release properties hold great potential for muscle and nerve regeneration.
基于聚乳酸（PLA）纳米纤维和来自聚氨酯 - 脲共聚物的电活性纳米颗粒制备了具有缓释性能的纳米纤维电活性复合支架，用于组织工程应用。将电活性聚氨酯 - 脲共聚物制成纳米颗粒，其对疏水性和亲水性药物均具有缓释动力学。通过电喷雾/静电纺丝工艺获得复合支架，其中电活性聚氨酯 - 脲纳米颗粒普遍包覆在PLA纳米纤维上。通过扫描电子显微镜观察纳米颗粒、PLA纤维和复合支架的形态。以布洛芬和芦丁为模型药物研究复合支架的药物释放曲线。研究了药物缓释的机制。负载芦丁的复合材料用作L929成纤维细胞增殖的活性支架。制备了电活性复合PLA纳米纤维支架的随机和排列结构。研究了复合支架表面化学和形貌线索对C2C12细胞增殖和分化的影响，发现复合纳米纤维的电活性和排列形态对C2C12成肌细胞增殖和分化具有协同作用。这些具有缓释性能的电活性纳米纤维复合支架在肌肉和神经再生方面具有巨大潜力。
