Zhu Dewei, Liu Maixian, Liu Xin, Liu Yang, Prasad Paras N, Swihart Mark T
Department of Chemical and Biological Engineering, University at Buffalo, The State University of New York, Buffalo, New York 14260-4200, USA.
J Mater Chem B. 2017 Jul 7;5(25):4934-4942. doi: 10.1039/c7tb01004d. Epub 2017 Jun 2.
In this study, we show that Au-CuSe heterogeneous nanocrystals have great promise for use in photothermal therapy (PTT). Ligand-stabilized heterogeneous gold-copper selenide (Au-CuSe) hybrid nanocrystals were synthesized by a colloidal gold seed-mediated method. The nanocrystals exhibit broad localized surface plasmon resonance (LSPR) across visible and near-infrared (NIR) wavelengths, arising from interactions between the two nanocrystal domains. After a ligand-exchange process, the NCs readily disperse in water while retaining their LSPR absorbance. Upon illumination with a 980 nm laser, the Au-CuSe nanocrystals produced significant photothermal heating with a photothermal transduction efficiency comparable to that of larger gold nanostructures that have been widely studied for PTT. In vitro photothermal heating of Au-CuSe nanocrystals in the presence of human cervical cancer cells caused cell ablation after 10 min laser irradiation. Cell viability assays demonstrated that the hybrid nanocrystals are biocompatible at doses needed for photothermal therapy. Overall, these heterogeneous nanocrystals provide the NIR PTT efficacy of larger gold nanorods in a much smaller overall nanostructure that may have advantages with respect to biodistribution and clearance.
在本研究中，我们表明金-硒化铜异质纳米晶体在光热疗法（PTT）中具有巨大的应用前景。通过胶体金种子介导法合成了配体稳定的异质金-铜硒（Au-CuSe）混合纳米晶体。这些纳米晶体在可见光和近红外（NIR）波长范围内表现出宽泛的局域表面等离子体共振（LSPR），这是由两个纳米晶域之间的相互作用引起的。经过配体交换过程后，纳米晶体易于分散在水中，同时保留其LSPR吸光度。在用980nm激光照射时，金-硒化铜纳米晶体产生显著的光热加热，其光热转换效率与已被广泛研究用于光热疗法的较大金纳米结构相当。在人宫颈癌细胞存在的情况下，对金-硒化铜纳米晶体进行体外光热加热，在激光照射10分钟后导致细胞消融。细胞活力测定表明，在光热疗法所需剂量下，混合纳米晶体具有生物相容性。总体而言，这些异质纳米晶体在更小的整体纳米结构中提供了与较大金纳米棒相当的近红外光热疗法疗效，这在生物分布和清除方面可能具有优势。
