Department of Biochemistry and Toxicology, University of Life Sciences, 20-950, Lublin, Poland.
Department of Poultry Science, University of Warmia and Mazury in Olsztyn, 10-719, Olsztyn, Poland.
Vet Res. 2020 Apr 7;51(1):50. doi: 10.1186/s13567-020-00776-y.
Two experiments were performed to investigate the effect of different ratios of arginine (Arg) to lysine (Lys) in diets with low (30% Lys; Experiment 1) and high (45% Lys; Experiment 2) methionine (Met) levels on selected metabolic parameters, oxidative and epigenetic DNA damage, and the mechanisms underlying intestinal barrier integrity in turkeys challenged with Clostridium perfringens. In each experiment, 108 one-day-old Hybrid Converter female turkeys were placed in 6 pens (18 birds per pen) and reared for 42 days. At 34, 36 and 37 days of age, half of the birds were subjected to C. perfringens challenge. A 3 × 2 factorial design with three levels of Arg relative to Lys (90, 100 and 110%; Arg, Arg and Arg, respectively) and C. perfringens infection (-, +) was employed. Challenging birds with C. perfringens increased lipid oxidation and the oxidation and methylation of DNA of intestinal mucosa, and down-regulated the activities of DNA-repairing enzymes. Neither the dietary treatment nor the challenge affected the markers of liver function or metabolism. Arg diets with the high Met level induced DNA oxidation and methylation whereas these processes were downregulated in birds fed Arg diets. The results indicate that Arg diets with high Met levels have a beneficial influence on the indicators of intestinal barrier integrity in turkeys with necrotic enteritis (NE). Despite the analyzed amino acid ratios interacted with the systems responsible for the maintenance of gut integrity in the host organism, this dietary intervention probably enabled birds to cope with NE.
两项实验研究了低（30%Lys；实验 1）和高（45%Lys；实验 2）蛋氨酸（Met）水平日粮中不同精氨酸（Arg）与赖氨酸（Lys）比例对选择代谢参数、氧化和表观遗传 DNA 损伤以及火鸡感染产气荚膜梭菌后肠道屏障完整性相关机制的影响。在每个实验中，将 108 只 1 日龄 Hybrid Converter 雌性火鸡置于 6 个围栏（每个围栏 18 只）中，饲养 42 天。在 34、36 和 37 日龄时，一半的鸟类接受产气荚膜梭菌挑战。采用 Arg 与 Lys 比值（90、100 和 110%；Arg、Arg 和 Arg）和产气荚膜梭菌感染（-、+）的 3×2 因子设计。用产气荚膜梭菌挑战鸟类增加了脂质氧化和肠黏膜 DNA 的氧化和甲基化，并下调了 DNA 修复酶的活性。日粮处理或挑战均未影响肝功能或代谢标志物。高 Met 水平的 Arg 日粮诱导了 DNA 氧化和甲基化，而在饲喂 Arg 日粮的鸟类中，这些过程被下调。结果表明，高 Met 水平的 Arg 日粮对坏死性肠炎（NE）火鸡肠道屏障完整性的指标有有益影响。尽管分析的氨基酸比例与宿主组织中维持肠道完整性的系统相互作用，但这种饮食干预可能使鸟类能够应对 NE。
