University of Naples Federico II, Department of Chemical, Materials and Production Engineering (DICMaPI), P.le Tecchio 80, 80125, Naples, Italy.
Fondazione Istituto Italiano di Tecnologia, IIT, Largo Barsanti e Matteucci 53, 80125, Naples, Italy.
Sci Rep. 2020 Apr 7;10(1):6028. doi: 10.1038/s41598-020-63234-x.
The combination of different imaging modalities can allow obtaining simultaneously morphological and functional information providing a more accurate diagnosis. This advancement can be reached through the use of multimodal tracers, and nanotechnology-based solutions allow the simultaneous delivery of different diagnostic compounds moving a step towards their safe administration for multimodal imaging acquisition. Among different processes, nanoprecipitation is a consolidate method for the production of nanoparticles and its implementation in microfluidics can further improve the control over final product features accelerating its potential clinical translation. A Hydrodynamic Flow Focusing (HFF) approach is proposed to produce through a ONE-STEP process Multimodal Pegylated crosslinked Hyaluronic Acid NanoParticles (PEG-cHANPs). A monodisperse population of NPs with an average size of 140 nm is produced and Gd-DTPA and ATTO488 compounds are co-encapsulated, simultaneously. The results showed that the obtained multimodal nanoparticle could work as MRI/Optical imaging probe. Furthermore, under the Hydrodenticity effect, a boosting of the T1 values with respect to free Gd-DTPA is preserved.
不同成像模式的结合可以同时获得形态和功能信息，从而提供更准确的诊断。这一进展可以通过使用多模态示踪剂来实现，而基于纳米技术的解决方案可以同时递送至不同的诊断化合物，朝着它们的安全管理用于多模态成像采集迈进了一步。在不同的过程中，纳米沉淀是生产纳米颗粒的一种成熟方法，其在微流控中的实施可以进一步改善对最终产品特性的控制，加速其潜在的临床转化。提出了一种流体动力学聚焦（Hydrodynamic Flow Focusing，HFF）方法来通过一步法生产多模态聚乙二醇交联透明质酸纳米颗粒（PEG-cHANPs）。通过该方法制备了具有 140nm 平均粒径的单分散纳米颗粒，并同时共包封了 Gd-DTPA 和 ATTO488 化合物。结果表明，所得到的多模态纳米颗粒可用作 MRI/光学成像探针。此外，在 Hydrodenticity 效应下，与游离 Gd-DTPA 相比，T1 值得到了增强。
