Zhou Jiuyun, Song Quanbin, Liu Xijuan, Ye Hongli, Wang Yusheng, Zhang Lan, Peng Shengjun, Qin Hongping
Department of Urology, The First Hospital of The Chinese People's Liberation Army, Lanzhou, Gansu 730000, P.R. China.
Department of Orthopedics, Gansu People's Hospital, Lanzhou, Gansu 730000, P.R. China.
Oncol Lett. 2020 May;19(5):3425-3430. doi: 10.3892/ol.2020.11464. Epub 2020 Mar 16.
Long non-coding (lnc) RNA Erbb4-IR has been associated with diabetic renal injury; however, its roles in other diseases remain unknown. Therefore, the present study investigated the involvement of Erbb4-IR in prostate carcinoma. Reverse transcription-quantitative PCR was used to analyze gene expression in tissue samples collected from patients with prostate carcinoma. Overexpression experiments via cell transfection were performed to determine the association between Erbb4-IR and microRNA (miR)-21. Furthermore, Cell Counting Kit-8 and cell apoptosis assays were performed to assess cell proliferation and apoptotic rate, respectively. The results revealed that Erbb4-IR was downregulated in prostate carcinoma tissues compared with adjacent non-cancerous tissues, and that low expression of Erbb4-IR in tumor tissues was closely associated with poor survival. Furthermore, miR-21 was upregulated in prostate carcinoma tissues compared with adjacent non-cancerous tissues and was inversely associated with Erbb4-IR expression in tumor tissues. cell experiments revealed that Erbb4-IR overexpression resulted in the downregulation of miR-21, while miR-21 overexpression did not significantly affect the expression of Erbb4-IR. Moreover, Erbb4-IR overexpression increased apoptosis and inhibited the proliferation of prostate carcinoma cells. miR-21 overexpression resulted in the opposite effect and attenuated the effects of Erbb4-IR overexpression. Therefore, the results of the present study suggested that lncRNA Erbb4-IR is downregulated in prostate carcinoma and may inhibit cancer development by downregulating miR-21.
长链非编码（lnc）RNA Erbb4-IR与糖尿病肾损伤有关；然而，其在其他疾病中的作用仍不清楚。因此，本研究调查了Erbb4-IR在前列腺癌中的作用。采用逆转录定量PCR分析前列腺癌患者组织样本中的基因表达。通过细胞转染进行过表达实验，以确定Erbb4-IR与微小RNA（miR）-21之间的关联。此外，分别进行细胞计数试剂盒-8和细胞凋亡检测，以评估细胞增殖和凋亡率。结果显示，与相邻非癌组织相比，前列腺癌组织中Erbb4-IR表达下调，且肿瘤组织中Erbb4-IR低表达与不良生存密切相关。此外，与相邻非癌组织相比，前列腺癌组织中miR-21上调，且与肿瘤组织中Erbb4-IR表达呈负相关。细胞实验显示，Erbb4-IR过表达导致miR-21下调，而miR-21过表达对Erbb4-IR表达无显著影响。此外，Erbb4-IR过表达增加了前列腺癌细胞的凋亡并抑制其增殖。miR-21过表达则产生相反的效果，并减弱了Erbb4-IR过表达的作用。因此，本研究结果表明，lncRNA Erbb4-IR在前列腺癌中表达下调，可能通过下调miR-21来抑制癌症发展。
