Cell Regulation Laboratory, Bionics Program, Tokyo University of Technology, Graduate School of Bionics, Computer and Media Science, 1404-1 Katakura, Hachioji, Tokyo, 192-0982, Japan.
Frontier Research Center, POLA Chemical Industries Inc, 560 Kashio-cho, Totsuka-ku, Yokohama, Kanagawa, 244-0812, Japan.
Int J Cosmet Sci. 2020 Aug;42(4):359-368. doi: 10.1111/ics.12622. Epub 2020 Jun 11.
Few histological studies have directly examined age-related changes within the lips, although non-invasive investigations of such changes are increasing. Therefore, this study aimed to provide histological and molecular data on age-dependent alterations in the vermilion.
Upper vermilion specimens from 15 female Caucasian cadavers (age range, 27-78 years) were investigated histologically or immunohistochemically.
Histologically, age-dependent decreases in areas occupied by hyaluronan and collagenous fibres in the dermis of upper vermilion were demonstrated. Elastic fibre content varied widely between individuals. The area occupied by muscle fibres in the orbicularis oris muscle region within the vermilion also correlated negatively with age. Immunohistochemically, signals of four proteins were attenuated in vermilion from older individuals compared with young individuals: procollagen type I, hyaluronan synthase (HAS)1, myosin heavy chain (MYH)2 (a component of fast-twitch oxidative muscle fibres) and MYH7 (a component of slow-twitch muscle fibres). In contrast, signals of cell migration inducing hyaluronidase 1 (CEMIP) were intensified in vermilion from older individuals. No marked differences between young and older individuals were seen in procollagen type III, HAS2, HAS3, hyaluronidase (HYAL)1, HYAL2, MYH1 or MYH4.
Age-dependent decreases of hyaluronan in the dermis of vermilion were prominent, possibly due to both the decrease in synthesis (HAS1) and the increase in degradation (CEMIP). Furthermore, age-dependent decreases in collagenous fibres and two types of muscle fibre in the vermilion were also identified histologically. Type I collagen, MYH2 and MYH7 appear to represent the molecules responsible for these respective decrements.
尽管针对唇部衰老变化的非侵入性研究越来越多，但鲜有组织学研究直接对其进行检测。因此，本研究旨在提供关于唇红组织中与年龄相关变化的组织学和分子数据。
对 15 名女性白种人尸体（年龄 27-78 岁）的上唇红唇组织进行组织学或免疫组织化学检查。
组织学上，上唇真皮中透明质酸和胶原纤维的面积随年龄的增长而减少。弹性纤维含量在个体之间差异很大。唇红肌肉纤维区的口轮匝肌纤维面积也与年龄呈负相关。免疫组织化学检查显示，与年轻人相比，老年人唇红组织中四种蛋白的信号减弱：Ⅰ型前胶原、透明质酸合酶 1（HAS1）、肌球蛋白重链（MYH）2（快肌氧化纤维的组成部分）和 MYH7（慢肌纤维的组成部分）。相比之下，在老年人的唇红组织中，细胞迁移诱导透明质酸酶 1（CEMIP）的信号增强。在年轻人和老年人之间，Ⅲ型前胶原、HAS2、HAS3、透明质酸酶 1（HYAL）1、HYAL2、MYH1 或 MYH4 之间无明显差异。
唇红真皮中透明质酸的年龄依赖性减少较为显著，这可能是由于 HAS1 合成减少和 CEMIP 降解增加所致。此外，唇红组织中胶原纤维和两种类型的肌肉纤维也随年龄增长而减少。Ⅰ型胶原、MYH2 和 MYH7 可能是导致这些相应减少的分子。
