Feng Linyan, Xu Liangxiong, Li Xiaojie, Xue Jinghua, Li Taotao, Duan Xuewu
Guangdong Provincial Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China.
School of Life Sciences, Huizhou University, Huizhou 510607, China.
J Fungi (Basel). 2022 Jan 25;8(2):111. doi: 10.3390/jof8020111.
Blue mold caused by is one of the most serious postharvest diseases of citrus fruit. The aim of this study was to investigate the inhibitory effect of a novel oligosaccharide ester, 6---L-mannopyranosyl-3--(2-methylbutanoyl)-4--(8-methyldecanoyl)-2--(4-methyl-hexanoyl) trehalose (MTE-1), against . Scanning electron microscopy (SEM) and transmission electron microscopy (TEM), along with transcriptome and proteome analysis also, were conducted to illuminate the underlying mechanism. Results showed that MTE-1 significantly inhibited growth in vitro in a dose-dependent manner. Moreover, MTE-1 suppressed the disease development of citrus fruit inoculated with . Furthermore, ultrastructure observation, as well as transcriptome and proteome analysis, indicated that MTE-1 treatment damaged the cell wall and plasma membrane in spores and mycelia of . In addition, MTE-1 regulated genes or proteins involved in primary metabolism, cell-wall metabolism, and pathogenicity. These results demonstrate that MTE-1 inhibited by damaging cell walls and membranes and disrupting normal cellular metabolism. These findings contribute to the understanding of the possible molecular action of MTE-1. Finally, MTE-1 also provides a new natural strategy for controlling diseases in postharvest fruit.
由[病原体名称未给出]引起的青霉病是柑橘类水果最严重的采后病害之一。本研究的目的是探究一种新型寡糖酯，6---L-吡喃甘露糖基-3--(2-甲基丁酰基)-4--(8-甲基癸酰基)-2--(4-甲基己酰基)海藻糖（MTE-1）对[病原体名称未给出]的抑制作用。同时进行了扫描电子显微镜（SEM）和透射电子显微镜（TEM）观察以及转录组和蛋白质组分析，以阐明其潜在机制。结果表明，MTE-1在体外以剂量依赖的方式显著抑制[病原体名称未给出]的生长。此外，MTE-1抑制了接种[病原体名称未给出]的柑橘类水果的病害发展。此外，超微结构观察以及转录组和蛋白质组分析表明，MTE-1处理破坏了[病原体名称未给出]孢子和菌丝体的细胞壁和质膜。此外，MTE-1调节了参与初级代谢、细胞壁代谢和致病性的基因或蛋白质。这些结果表明，MTE-1通过破坏细胞壁和细胞膜以及扰乱正常细胞代谢来抑制[病原体名称未给出]。这些发现有助于理解MTE-1可能的分子作用。最后，MTE-1也为控制采后果实病害提供了一种新的天然策略。
