Chen Chuying, Qi Wenwen, Peng Xuan, Chen Jinyin, Wan Chunpeng
Jiangxi Key Laboratory for Postharvest Technology and Nondestructive Testing of Fruits & Vegetables, Collaborative Innovation Center of Postharvest Key Technology and Quality Safety of Fruits and Vegetables, Jiangxi Agricultural University, Nanchang 330045, China.
Pingxiang University, Pingxiang 337055, China.
Microorganisms. 2019 Jan 26;7(2):36. doi: 10.3390/microorganisms7020036.
7-demethoxytylophorine (DEM) is a phenanthroindolizidine alkaloid, which is reported to be effective in inhibiting leucocytes and regulation of human immunity. However, few studies reported the inhibitory effect of DEM against plant-pathogenic fungi, particularly postharvest pathogen (). Current studies have investigated the antifungal activity of DEM through membrane damage and energy deficit in . The results showed that the DEM potentially inhibits the growth of in a dose-dependent manner. In vitro (mycelial growth and spore germination) tests showed great minimal inhibitory concentration (MIC) (1.56 µg mL) and minimum fugicide concentration (MFC) (6.25 µg mL). Microscopic analyses showed that mycelial morphology of was severely damaged following DEM treatment. Moreover, relative electrical conductivity and lysis ability assays showed that DEM treatment aids in destroying the integrity of plasma membranes that deplete reducing sugars and soluble proteins. The activity of malate dehydrogenase (MDH) and succinate dehydrogenase (SDH) demonstrated that DEM led to the disruption of TCA cycle in mycelia. The results of this study led us to conclude that, DEM could be used as a natural antifungal agent for controlling postharvest blue mold disease of citrus fruits caused by .
7-去甲氧基娃儿藤碱（DEM）是一种菲并吲哚里西啶生物碱，据报道其在抑制白细胞和调节人体免疫力方面具有功效。然而，鲜有研究报道DEM对植物病原真菌，尤其是采后病原菌（此处括号内容缺失）的抑制作用。目前的研究通过膜损伤和能量缺乏来探究DEM的抗真菌活性。结果表明，DEM可能以剂量依赖的方式抑制（此处括号内容缺失）的生长。体外（菌丝体生长和孢子萌发）试验显示其最低抑菌浓度（MIC）（1.56微克/毫升）和最低杀菌浓度（MFC）（6.25微克/毫升）很高。显微镜分析表明，DEM处理后（此处括号内容缺失）的菌丝体形态受到严重破坏。此外，相对电导率和裂解能力测定表明，DEM处理有助于破坏耗尽还原糖和可溶性蛋白质的质膜完整性。苹果酸脱氢酶（MDH）和琥珀酸脱氢酶（SDH）的活性表明，DEM导致（此处括号内容缺失）菌丝体中三羧酸循环的破坏。本研究结果使我们得出结论，DEM可作为一种天然抗真菌剂，用于控制由（此处括号内容缺失）引起的柑橘类水果采后青霉病。
