Kim Chloe, Hoffmann Gwendolyn, Searson Peter C
Department of Materials Science and Engineering and Institute for Nanobiotechnology, Johns Hopkins University , 3400 North Charles Street, Baltimore, Maryland 21218, United States.
ACS Sens. 2017 Jun 23;2(6):766-772. doi: 10.1021/acssensors.7b00119. Epub 2017 Jun 12.
Malaria persists as a disease of high morbidity and mortality due to improper diagnosis, overuse of drugs, rapidly evolving drug resistant parasites, and poor disease monitoring. The two common tests used in developing countries, microscopic examination of Glemsa slides and rapid diagnostic tests (RDTs), have limitations associated with variability in specificity and sensitivity, and qualitative outcome. Here we report on an immunoassay using magnetic beads for capture and quantum dots for detection of histidine-rich protein 2 (HRP2). Conventional immunoassays, such as ELISA, and molecular analysis tools, such as PCR, are difficult to implement in low resource settings. Therefore, to provide a proof-of-principle of translation of this assay to low resource settings, we demonstrate HRP2 detection in an automated droplet-based microfluidic device. Droplet-based platforms have the potential to allow translation of molecular detection assays to point-of-care use in low resource settings.
由于诊断不当、药物滥用、寄生虫耐药性快速演变以及疾病监测不力，疟疾仍然是一种发病率和死亡率很高的疾病。发展中国家常用的两种检测方法，即吉姆萨涂片显微镜检查和快速诊断检测（RDT），存在特异性和敏感性变异性以及定性结果方面的局限性。在此，我们报告一种使用磁珠进行捕获、量子点进行富含组氨酸蛋白2（HRP2）检测的免疫测定法。传统免疫测定法（如ELISA）和分子分析工具（如PCR）在资源匮乏地区难以实施。因此，为了证明该检测方法在资源匮乏地区的转化可行性，我们在基于液滴的自动化微流控装置中展示了HRP2检测。基于液滴的平台有可能将分子检测方法转化为在资源匮乏地区的即时检测应用。
