Kumbar Vijay M, Peram Malleswara Rao, Kugaji Manohar S, Shah Tejas, Patil Sanjivani P, Muddapur Uday M, Bhat Kishore G
Central Research Laboratory, Maratha Mandal's Nathajirao G. Halgekar Institute of Dental Sciences and Research Centre, Near KSRP Ground, Bauxite road, Belgaum, Karnataka, 590 010, India.
Department of Pharmaceutics, Maratha Mandal's College of Pharmacy, Belgaum, Karnataka, 590 010, India.
Odontology. 2021 Jan;109(1):18-28. doi: 10.1007/s10266-020-00514-y. Epub 2020 Apr 11.
Porphyromonas gingivalis is a keystone pathogen and major colonizer in host tissue which plays a pivotal role in periodontitis among the other polymicrobial infections. Increasing facts demonstrate that curcumin has antibacterial activity and anti-biofilm effect against the periodontopathogens through diverse mechanisms that have a positive impact on periodontal health. The present study was aimed to elucidate the effect of curcumin on biofilm formation and virulence factor gene expression of P. gingivalis. By using gene expression studies, we exploited the mechanism of anti-biofilm effects of curcumin on P. gingivalis. The minimum inhibitory concentration and minimum bactericidal concentration of curcumin for both ATCC and clinical strains of P. gingivalis were found to be 62.5 and 125 µg ml respectively. Curcumin prevented bacterial adhesion and biofilm formation in a dose-dependent manner. Further, curcumin attenuated the virulence of P. gingivalis by reducing the expression of genes coding for major virulence factors, including adhesions (fimA, hagA, and hagB) and proteinases (rgpA, rgpB, and kgp). The results indicated that curcumin has shown anti-biofilm as well as antibacterial activity against P. gingivalis. Further, curcumin because of its pleiotropic actions could be a simple and inexpensive therapeutic strategy in the treatment of periodontal disease.
牙龈卟啉单胞菌是宿主组织中的关键病原体和主要定植菌，在包括牙周炎在内的多种微生物感染中起关键作用。越来越多的事实表明，姜黄素通过多种机制对牙周病原体具有抗菌活性和抗生物膜作用，对牙周健康产生积极影响。本研究旨在阐明姜黄素对牙龈卟啉单胞菌生物膜形成和毒力因子基因表达的影响。通过基因表达研究，我们探究了姜黄素对牙龈卟啉单胞菌抗生物膜作用的机制。发现姜黄素对牙龈卟啉单胞菌的ATCC菌株和临床菌株的最低抑菌浓度和最低杀菌浓度分别为62.5和125 μg/ml。姜黄素以剂量依赖的方式阻止细菌黏附和生物膜形成。此外，姜黄素通过降低编码主要毒力因子的基因表达，包括黏附素（fimA、hagA和hagB）和蛋白酶（rgpA、rgpB和kgp），减弱了牙龈卟啉单胞菌的毒力。结果表明，姜黄素对牙龈卟啉单胞菌具有抗生物膜和抗菌活性。此外，由于姜黄素具有多种作用，它可能是治疗牙周疾病的一种简单且廉价的治疗策略。
