Lermen Dominik, Gwinner Frederik, Bartel-Steinbach Martina, Mueller Sabine C, Habermann Jens K, Balwir Matharoo-Ball, Smits Elke, Virgolino Ana, Fiddicke Ulrike, Berglund Marika, Åkesson Agneta, Bergstrom Anna, Leander Karin, Horvat Milena, Snoj Tratnik Janja, Posada de la Paz Manuel, Castaño Calvo Argelia, Esteban López Marta, von Briesen Hagen, Zimmermann Heiko, Kolossa-Gehring Marike
Fraunhofer Institute for Biomedical Engineering IBMT, Biomonitoring & Biobanks, Sulzbach, Germany.
European, Middle Eastern & African Society for Biopreservation and Biobanking, Brussels, Belgium.
Biopreserv Biobank. 2020 Apr;18(2):122-135. doi: 10.1089/bio.2019.0092.
Human biomonitoring (HBM) depends on high-quality human samples to identify status and trends in exposure and ensure comparability of results. In this context, much effort has been put into the development of standardized processes and quality assurance for sampling and chemical analysis, while effects of sample storage and shipment on sample quality have been less thoroughly addressed. To characterize the currently applied storage and shipment procedures within the consortium of the European Human Biomonitoring Initiative (HBM4EU), which aims at harmonization of HBM in Europe, a requirement analysis based on data from an online survey was conducted. In addition, the online survey was addressed to professionals in clinical biobanking represented by members of the European, Middle Eastern and African Society for Biopreservation and Biobanking (ESBB) to identify the current state-of-the-art in terms of sample storage and shipment. Results of this survey conducted in these two networks were compared to detect processes with potential for optimization and harmonization. In general, many similarities exist in sample storage and shipment procedures applied by ESBB members and HBM4EU partners and many requirements for ensuring sample quality are already met also by HBM4EU partners. Nevertheless, a need for improvement was identified for individual steps in sample storage, shipment, and related data management with potential impact on sample and data quality for HBM purposes. Based on these findings, recommendations for crucial first steps to further strengthen sample quality, and thus foster advancement in HBM on a pan-European level are given.
人体生物监测（HBM）依赖高质量的人体样本，以识别暴露状况和趋势，并确保结果的可比性。在此背景下，人们在采样和化学分析的标准化流程及质量保证方面投入了大量精力，而样本储存和运输对样本质量的影响却较少得到充分探讨。为了描述欧洲人体生物监测倡议（HBM4EU）联盟目前应用的储存和运输程序（该联盟旨在协调欧洲的人体生物监测工作），基于在线调查数据进行了需求分析。此外，在线调查还面向由欧洲、中东和非洲生物保存与生物银行协会（ESBB）成员代表的临床生物样本库专业人员，以确定样本储存和运输方面的当前技术水平。对在这两个网络中进行的这项调查结果进行了比较，以发现具有优化和协调潜力的流程。总体而言，ESBB成员和HBM4EU合作伙伴应用的样本储存和运输程序存在许多相似之处，HBM4EU合作伙伴也已经满足了许多确保样本质量的要求。然而，已确定在样本储存、运输及相关数据管理的各个步骤中需要改进，这些改进可能会对用于HBM目的的样本和数据质量产生影响。基于这些发现，给出了关于进一步加强样本质量的关键首要步骤的建议，从而推动泛欧洲层面HBM的发展。
