Kim Seon Hwa, Vujanovic Vladimir
Department of Food and Bioproduct Sciences, University of Saskatchewan, 51 Campus Drive, Saskatoon, SK S7N 5A8 Canada.
Bioresour Bioprocess. 2021;8(1):127. doi: 10.1186/s40643-021-00479-y. Epub 2021 Dec 16.
Mycoparasites are an assemblage of biotrophic and necrotrophic fungi that occur on plant pathogenic fungal hosts. Biotrophic mycoparasites are often overlooked in transcriptomic-based biocontrol studies. () is a specific biotrophic mycoparasite of plant pathogenic , a devastating Fusarium head blight (FHB) disease in small-grain cereals. To understand the biotrophic mycoparasitism comprehensively, we performed Illumina RNA-Seq transcriptomic study on the fungus-fungus interaction in vitro. The aim is to identify the transcript-level mechanism related to the biotrophic mycoparasitism, particularly its ability to effectively control the 3-ADON chemotype. A shift in the transcriptomic profile of the mycoparasite was triggered in response to its interaction with during recognition (1.5 days) and colonization (3.5 days) steps. RNA-Seq analysis revealed ~ 30% of annotated transcripts with "function unknown". Further, 14 differentially expressed genes functionally linked to the biotrophic mycoparasitism were validated by quantitative real-time PCR (qPCR). The gene expression patterns of the filamentous haemagglutinin/adhesin/attachment factor as well as cell wall-degrading glucanases and chitinases were upregulated by host interaction. Besides, mycoparasitism-associated antioxidant resistance genes encoding ATP-binding cassette (ABC) transporter(s) and glutathione synthetase(s) were upregulated. However, the thioredoxin reductase was downregulated which infers that this antioxidant gene can be used as a resistance marker to assess antifungal and antimycotoxigenic activities. The interactive transcriptome of provides new insights into specific mycoparasitism and will contribute to future research in controlling FHB.
菌寄生真菌是一类寄生于植物病原真菌宿主上的生物营养型和坏死营养型真菌的集合。在基于转录组学的生物防治研究中，生物营养型菌寄生真菌常常被忽视。（）是植物病原菌（一种在小粒谷物中引发毁灭性赤霉病（FHB）的病原菌）的一种特定生物营养型菌寄生真菌。为了全面了解生物营养型菌寄生现象，我们对体外真菌 - 真菌相互作用进行了Illumina RNA测序转录组学研究。目的是确定与生物营养型菌寄生相关的转录水平机制，特别是其有效控制3 - ADON化学型的能力。在识别（1.5天）和定殖（3.5天）步骤中，菌寄生真菌与（）相互作用时，其转录组谱发生了变化。RNA测序分析显示约30%的注释转录本“功能未知”。此外，通过定量实时PCR（qPCR）验证了14个与生物营养型菌寄生功能相关的差异表达基因。丝状血凝素/粘附素/附着因子以及细胞壁降解葡聚糖酶和几丁质酶的基因表达模式因宿主相互作用而上调。此外，编码ATP结合盒（ABC）转运蛋白和谷胱甘肽合成酶的与菌寄生相关的抗氧化抗性基因也上调。然而，硫氧还蛋白还原酶下调，这表明该抗氧化基因可作为评估（）抗真菌和抗产毒活性的抗性标记。（）的相互作用转录组为特定的菌寄生现象提供了新的见解，并将有助于未来控制赤霉病的研究。
