Hubei Provincial Key Laboratory for Applied Toxicology, Hubei Provincial Center for Disease Control and Prevention, Wuhan, 430079, China.
Hubei Provincial Key Laboratory for Applied Toxicology, Hubei Provincial Center for Disease Control and Prevention, Wuhan, 430079, China; Fuling District Center for Disease Control and Prevention, Chongqing, 408000, China.
Food Chem Toxicol. 2020 Jun;140:111324. doi: 10.1016/j.fct.2020.111324. Epub 2020 Apr 10.
In the present study, a novel transgenic rice line T1c-19 carrying cry1C* gene was evaluated in Sprague-Dawley (SD) rats by a 52-week feeding study, aiming at determining its unintended effects. The rice T1c-19 and its parental rice were prepared at a level of up to 60.75% in the growth diet and 66.75% in the maintenance diet, respectively. AIN-93 diet was used as a nutritional control. All the diets were nutritionally balanced. Each group, with 48 rats of both genders, was fed the corresponding diet for 52 weeks. The results of clinical signs, body weight and food consumption of the transgenic rice group were comparable to those of the parental rice group. Clinical measurements were made on weeks 13, 26 and 52, and statistical significances were observed in several hematological and serum biochemical indices between the two rice groups and were not considered as treatment-related. The terminal histopathological examination showed some spontaneous lesions in all groups with no significant difference among them. Taken together, the results of the present 52-week chronic toxicity study of transgenic rice T1c-19 exerted no unintended adverse effects on SD rats.
在本研究中，通过一项为期 52 周的喂养研究，评估了携带 cry1C*基因的新型转基水稻品系 T1c-19 在 Sprague-Dawley（SD）大鼠中的潜在影响。T1c-19 水稻及其亲本水稻分别以生长饲料中高达 60.75%和维持饲料中高达 66.75%的水平制备。AIN-93 饮食被用作营养对照。所有的饮食都是营养均衡的。每组雌雄各 48 只大鼠，分别用相应的饮食喂养 52 周。转基水稻组的临床症状、体重和食物消耗与亲本水稻组相似。在第 13、26 和 52 周进行临床测量，两个水稻组之间的几个血液学和血清生化指标存在统计学差异，但不被认为与处理有关。终端组织病理学检查显示所有组均有一些自发性病变，组间无明显差异。综上所述，T1c-19 转基水稻的为期 52 周的慢性毒性研究结果表明，该品系对 SD 大鼠没有产生非预期的不良影响。
