State Key Laboratory of Chemical Resource Engineering, Key Lab of Biomedical Materials of Natural Macromolecules, Beijing University of Chemical Technology, Ministry of Education, Beijing Laboratory of Biomedical Materials, Beijing University of Chemical Technology, Beijing 100029, China.
State Key Laboratory of Chemical Resource Engineering, Key Lab of Biomedical Materials of Natural Macromolecules, Beijing University of Chemical Technology, Ministry of Education, Beijing Laboratory of Biomedical Materials, Beijing University of Chemical Technology, Beijing 100029, China.
J Control Release. 2020 Jul 10;323:24-35. doi: 10.1016/j.jconrel.2020.04.014. Epub 2020 Apr 10.
Supramolecular hydrogels confer control over structural properties in a reversible, dynamic, and biomimetic fashion. The design of supramolecular hydrogels with an improved structural and functional recapitulation of damaged organs is important for clinical applications. For wound healing management, in particular, an effective healing process, through the modulation of epidermal growth factor (EGF) delivery using supramolecular polysaccharide hydrogels, has yet to be developed. In this study, photo-responsive supramolecular polysaccharide hydrogels were formed through host-guest interactions between azobenzene and β-cyclodextrin groups conjugated to hyaluronic acid chains. By exploiting the photoisomerization properties of azobenzene under different wavelengths, a supramolecular hydrogel featuring a dynamic spatial network crosslink density through the application of a light stimulus was obtained. Under ultra violet (UV) light, the loosened hydrogel can rapidly release EGF, thereby enhancing EGF delivery at the wound site. Based on an in vivo assessment of the healing process through a full-thickness skin defect model, the controlled EGF release from a supramolecular hydrogel exhibited superior wound healing efficiency with respect to granulation tissue formation, growth factor levels, and angiogenesis. Therefore, the proposed supramolecular hydrogels are potentially valuable as controlled delivery systems for future clinical wound healing applications.
超分子水凝胶以一种可恢复、动态和仿生的方式控制结构性质。设计具有改善的结构和功能的超分子水凝胶，以模拟受损器官，对于临床应用非常重要。特别是对于伤口愈合管理，通过使用超分子多糖水凝胶来调节表皮生长因子 (EGF) 的递送，还需要开发一种有效的愈合过程。在这项研究中，通过将偶氮苯和β-环糊精基团与透明质酸链缀合，形成了光响应的超分子多糖水凝胶。通过利用偶氮苯在不同波长下的光异构化性质，通过施加光刺激获得了具有动态空间网络交联密度的超分子水凝胶。在紫外 (UV) 光下，疏松的水凝胶可以迅速释放 EGF，从而增强伤口部位的 EGF 递送。通过全厚度皮肤缺损模型对愈合过程进行体内评估，超分子水凝胶中 EGF 的控制释放在肉芽组织形成、生长因子水平和血管生成方面表现出优越的伤口愈合效率。因此，所提出的超分子水凝胶有望成为未来临床伤口愈合应用的有价值的控制释放系统。
