Isolation and Characterization of a Novel Phage for Controlling Multidrug-Resistant .

The emergence of multidrug-resistant bacterial pathogens has severely threatened global health. A phage with the ability to efficiently and specifically lyse bacteria is considered an alternative for controlling multidrug-resistant bacterial pathogens. The discovery of novel agents for controlling the infections caused by is urgent due to the broad multidrug-resistance of . Only a few phage isolates have been reported to infect multidrug-resistant . In this study, by using the multidrug-resistant strain as an indicator, a novel phage called vB_KleS-HSE3, which maintains high antibacterial activity and high physical stability, was isolated from hospital sewage. This phage infected one of four tested multidrug-resistant strains. This phage belongs to the Siphoviridae family and a comparative genomic analysis showed that this phage is part of a novel phage lineage among the Siphoviridae family of phages that infect strains of . Based on its features, the vB_KleS-HSE3 phage has potential for controlling infections caused by multidrug-resistant