Wang Xue, Li Junjie, Guo Jing, Qiao Qian, Guo Xianfeng, Ma Yan
1College of Forestry, Shandong Agricultural University, No. 61, Daizong Road, 271018 Tai'an, Shandong China.
Shandong Provincial Research Center of Demonstration Engineering Technology for Urban and Rural Landscapes, 271018 Tai'an, Shandong China.
Hortic Res. 2020 Apr 1;7:57. doi: 10.1038/s41438-020-0267-7. eCollection 2020.
In this study, the disease resistance gene was isolated from the leaves of and analyzed by bioinformatics methods, and the localization of the encoded protein was explored. Quantitative real-time PCR (qRT-PCR) was also used to explore the response of this gene to . The results showed that the gene sequence contained multiple cis-acting elements involved in the response to hormone signaling molecules belonging to the IIe subgroup of the WRKY family, and the encoded proteins were located in the nucleus. The gene has a positive regulatory effect on infection. After silencing the gene via virus-induced gene silencing (VIGS), it was found that the gene-silenced plants were more sensitive to infection than the wild plants, exhibiting more severe infection symptoms and different degrees of changes in the expression of the pathogenesis-related (PR) genes. In addition, we showed that the endogenous jasmonic acid (JA) content of was increased in response to infection, whereas the salicylic acid (SA) content decreased. After gene silencing, the levels of the two hormones changed accordingly, indicating that , acting as a disease resistance-related transcriptional activator, exerts a regulatory effect on JA and SA signals. This study lays the foundation for functional research on WRKY genes in and for the discovery of candidate disease resistance genes.
在本研究中，从[植物名称]的叶片中分离出抗病基因，并通过生物信息学方法进行分析，同时探究了编码蛋白的定位。还利用定量实时荧光定量PCR（qRT-PCR）来探究该基因对[病原体名称]的响应。结果表明，该基因序列包含多个参与响应属于WRKY家族IIe亚组的激素信号分子的顺式作用元件，且编码蛋白定位于细胞核。[基因名称]基因对[病原体名称]感染具有正向调控作用。通过病毒诱导基因沉默（VIGS）使[基因名称]基因沉默后，发现基因沉默植株比野生植株对[病原体名称]感染更敏感，表现出更严重的感染症状以及病程相关（PR）基因表达的不同程度变化。此外，我们发现[植物名称]的内源茉莉酸（JA）含量在响应[病原体名称]感染时增加，而水杨酸（SA）含量降低。[基因名称]基因沉默后，这两种激素的水平相应改变，表明[基因名称]作为抗病相关转录激活因子，对JA和SA信号发挥调控作用。本研究为[植物名称]中WRKY基因的功能研究以及候选抗病基因的发现奠定了基础。
