Yang Liping, Wan Xin, Zhou Runyang, Yuan Yingdan
College of Horticulture and Landscape Architecture, Yangzhou University, Yangzhou 225009, China.
Jiangsu Academy of Forestry, Nanjing 211153, China.
Biology (Basel). 2023 Oct 24;12(11):1363. doi: 10.3390/biology12111363.
The composition and diversity of the rhizosphere microbial community maintain the stability of the root microclimate, and several studies have focused on this aspect of rhizosphere microorganisms. However, how these communities vary with cultivars of a species is not completely understood. -a perennial herb species of the family Paeoniaceae-includes a wide variety of cultivars, with rich rhizosphere microbial resources. Hence, we studied the differences in rhizosphere bacterial communities associated with eight cultivars. We noted that Actinobacteria, Proteobacteria, Acidobacteria, Bacteroidetes, Firmicutes, Verrucomicrobia, Planctomycetes and Chloroflexi were the dominant phyla associated with the cultivars. The composition of rhizosphere bacterial community of different cultivars was highly similar at taxonomic levels, but there were slightly differences in the relative abundance. LEfSe analysis showed that the cultivars "Sheng Tao Hua" and "Zi Lou Xian Jin" exhibited the most biomarkers. Differential ASV analysis revealed the maximum difference in ASV abundance between "Lian Tai" and "Zi Hong Zheng Hui", as well as between "Sheng Tao Hua" and "Tao Hua Fei Xue", and the maximum similarity between "Duo Ye Zi" and "Xue Feng". Co-occurrence network analysis revealed that rhizosphere bacteria in most cultivars maintain homeostasis by cooperation, wherein Actinobacteria and Proteobacteria played a vital role. In addition, microbial resources related to cultivars like bioremediation, organic degradation and resistance to diseases are found. This study revealed the structures of the rhizosphere bacterial communities associated with different cultivars of and explored their stress resistance potential, which can be used to guide future agricultural practices.
根际微生物群落的组成和多样性维持着根际微气候的稳定性，已有多项研究聚焦于根际微生物的这一方面。然而，这些群落如何随物种的品种而变化尚未完全清楚。芍药科的一种多年生草本植物——包含多种品种，拥有丰富的根际微生物资源。因此，我们研究了与八个品种相关的根际细菌群落差异。我们注意到放线菌门、变形菌门、酸杆菌门、拟杆菌门、厚壁菌门、疣微菌门、浮霉菌门和绿弯菌门是与这些品种相关的优势菌门。不同品种根际细菌群落的组成在分类水平上高度相似，但相对丰度略有差异。线性判别分析效应大小（LEfSe）分析表明，“盛桃花”和“紫楼仙锦”品种表现出最多的生物标志物。差异可操作分类单元（ASV）分析显示，“莲台”和“紫红正辉”之间以及“盛桃花”和“桃花飞雪”之间的ASV丰度差异最大，“多叶子”和“雪峰”之间的相似度最高。共现网络分析表明，大多数品种的根际细菌通过合作维持稳态，其中放线菌门和变形菌门发挥了至关重要的作用。此外，还发现了与生物修复、有机降解和抗病等品种相关的微生物资源。本研究揭示了与不同品种相关的根际细菌群落结构，并探索了它们的抗逆潜力，可用于指导未来的农业实践。
