β-氨基丁酸和白粉病感染增强了黄瓜（L.）中防御相关基因和水杨酸的激活。
β-Aminobutyric Acid and Powdery Mildew Infection Enhanced the Activation of Defense-Related Genes and Salicylic Acid in Cucumber ( L.).
机构信息
Division of Horticultural Biotechnology, School of Biotechnology, Hankyong National University, Anseong 17579, Republic of Korea.
Institute of Genetic Engineering, Hankyong National University, Anseong 17579, Republic of Korea.
出版信息
Genes (Basel). 2023 Nov 17;14(11):2087. doi: 10.3390/genes14112087.
Powdery mildew disease, caused by , is a major disease affecting cucumbers cultivated in greenhouses. This study was conducted to find defense genes induced by β-aminobutyric acid (BABA) and powdery mildew in cucumber. Disease severities of 25% and 5% were exhibited by the 2000 and 5000 mg/L BABA-treated cucumber, respectively. BABA did not affect the spore germination of the powdery mildew pathogen, showing that BABA is not an antifungal agent against the pathogen. In quantitative real-time PCR analysis, BABA-treated cucumber upregulated the transcriptional levels of the defense genes , , , , , Cs , , and in cucumber to maximum levels at 48 h, whereas reached maximum expression after 24 h, and further, salicylic acid (SA) levels were significantly increased in BABA-treated cucumber plants. In addition, the cucumber infected with powdery mildew underwent a 1.6- to 47.3-fold enhancement in the defense genes , , , , , , , and compared to heathy cucumber. These results suggest that the BABA-induced defense response is associated with SA signaling pathway-dependent systemic acquired resistance (SAR) in cucumber, which is involved in plant resistance mechanisms.
白粉病是由引起的，是一种严重影响温室黄瓜种植的病害。本研究旨在寻找β-氨基丁酸（BABA）和白粉病诱导的黄瓜防御基因。用 2000 和 5000mg/L BABA 处理的黄瓜，其病情分别达到 25%和 5%。BABA 并不影响白粉病病原菌的孢子萌发，表明 BABA 不是针对病原菌的抗真菌剂。在实时定量 PCR 分析中，BABA 处理的黄瓜中防御基因、、、、、、、和在 48 小时内上调至最大水平，而在 24 小时后达到最大表达水平，此外，BABA 处理的黄瓜中水杨酸（SA）水平显著增加。此外，与健康黄瓜相比，感染白粉病的黄瓜中防御基因、、、、、、、和的表达水平分别增强了 1.6-47.3 倍。这些结果表明，BABA 诱导的防御反应与黄瓜中依赖 SA 信号通路的系统性获得抗性（SAR）有关，这涉及植物的抗性机制。
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