Institute of Agricultural Resources and Regional Planning, CAAS, Beijing, 100081, China.
College of Bioscience and Engineering, Jiangxi Agricultural University, Nanchang, 330045, People's Republic of China.
Arch Microbiol. 2020 Sep;202(7):1687-1692. doi: 10.1007/s00203-020-01878-z. Epub 2020 Apr 13.
A Gram-staining positive, aerobic, non-motile, rod-shaped bacterium, designated strain LAM7114, was isolated from soil sample collected from a birch forest in Xinjiang Uygur Autonomous Region, China. The optimal temperature and pH for the growth of strain LAM7114 were 30 °C and 7.0, respectively. Strain LAM7114 could grow in the presence of NaCl up to 10% (w/v). Comparative analysis of the 16S rRNA gene sequences revealed that LAM7114 was closely related to the members of the family genus Streptomyces, with the highest similarity to Streptomyces urticae NEAU-PCY-1 (98.3%) and Streptomyces fildesensis GW25-5 (98.2%). The genomic G + C content was 70.0 mol%. The DNA-DNA hybridization values between strain LAM7114 and S. urticae CCTCC AA 2017015, S. fildesensis CGMCC 4.5735 were 32.5 ± 1.8% and 27.5 ± 2.6%, respectively. The cell wall contained LL-diaminopimelic acid as the diagnostic diamino acid in the peptidoglycan. The whole-cell hydrolysates included glucose and mannose. The major fatty acids were anteiso-C, iso-C and iso-C. The predominant menaquinones were MK-9(H), MK-9(H) and MK-9(H). The main polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, three unidentified aminophospholipids, three unidentified phospholipids, and an unidentified aminolipid. Based on the phenotypic characteristics and genotypic analyses, we propose that strain LAM7114 represents a novel species in the genus Streptomyces, for which the name Streptomyces soli sp. nov. is proposed. The type strain is LAM7114 (= CGMCC 4.7581 = JCM 32822).
一株革兰氏阳性、需氧、不运动的杆状细菌，被命名为 LAM7114 菌株，从中国新疆维吾尔自治区桦树林土壤样本中分离得到。LAM7114 菌株的最佳生长温度和 pH 值分别为 30°C 和 7.0。该菌株可以在 10%（w/v）的 NaCl 存在下生长。16S rRNA 基因序列的比较分析表明，LAM7114 与链霉菌属的成员密切相关，与 Streptomyces urticae NEAU-PCY-1（98.3%）和 Streptomyces fildesensis GW25-5（98.2%）的相似度最高。基因组 G+C 含量为 70.0 mol%。LAM7114 菌株与 S. urticae CCTCC AA 2017015、S. fildesensis CGMCC 4.5735 的 DNA-DNA 杂交值分别为 32.5±1.8%和 27.5±2.6%。细胞壁中含有 LL-二氨基庚二酸作为肽聚糖中的特征二氨基庚二酸。全细胞水解产物包括葡萄糖和甘露糖。主要脂肪酸为 anteiso-C、iso-C 和 iso-C。主要的menaquinones 为 MK-9(H)、MK-9(H)和 MK-9(H)。主要的极性脂为双磷脂酰甘油、磷脂酰乙醇胺、磷脂酰肌醇、三种未鉴定的氨基磷脂、三种未鉴定的磷脂和一种未鉴定的氨基脂。基于表型特征和基因型分析，我们建议 LAM7114 菌株代表链霉菌属中的一个新种，命名为 Soli 土壤链霉菌（Streptomyces soli）。模式菌株为 LAM7114（=CGMCC 4.7581=JCM 32822）。
