基于连接-滚环扩增的 miRNA 均相多重数字检测。
Homogeneous multiplexed digital detection of microRNA with ligation-rolling circle amplification.
机构信息
Department of Chemistry, Tsinghua University, Beijing 100084, China.
出版信息
Chem Commun (Camb). 2020 May 19;56(40):5409-5412. doi: 10.1039/d0cc01530j.
PMID:32286590
Abstract
A simple multiplexed digital microRNA detection strategy with fluorescence flow cytometry was proposed. By isothermal ligation-rolling circle amplification, multiplexed microRNAs could be simultaneously converted to a series of nanoflower balls (NFBs) and counted by flow cytometry directly.
摘要
提出了一种简单的荧光流式细胞术多重数字微 RNA 检测策略。通过等温连接-滚环扩增，可将多重微 RNA 同时转化为一系列纳米花球（NFB），并直接通过流式细胞术进行计数。
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