State Key Laboratory of Marine Resource Utilization in South China Sea, College of Materials Science and Engineering, Hainan University, Haikou 570228, China.
Sensors (Basel). 2020 Apr 3;20(7):2017. doi: 10.3390/s20072017.
MicroRNAs regulate and control the growth and development of cells and can play the role of oncogenes and tumor suppressor genes, which are involved in the occurrence and development of cancers. In this study, DNA fragments obtained by target-induced rolling-circle amplification were constructed to complement with self-cleaving deoxyribozyme (DNAzyme) and release fluorescence biomolecules. This sensing approach can affect multiple signal amplification permitting fluorescence detection of microRNAs at the pmol L level hence affording a simple, highly sensitive, and selective low cost detection platform.
MicroRNAs 调节和控制细胞的生长和发育，可以作为癌基因和肿瘤抑制基因发挥作用，参与癌症的发生和发展。在这项研究中，构建了通过靶向诱导滚环扩增获得的 DNA 片段，以补充自我切割脱氧核酶（DNAzyme）并释放荧光生物分子。这种传感方法可以影响多种信号放大，从而在 pmol L 水平上进行 microRNAs 的荧光检测，因此提供了一种简单、高灵敏、高选择性和低成本的检测平台。
