Institute of Analytical Chemistry, Department of Chemistry, Zhejiang University, Hangzhou, 310058, P. R. China.
Chempluschem. 2020 Apr;85(4):725-733. doi: 10.1002/cplu.202000145.
Cellular heterogeneity is always present in any cell population. It is the fundamental to understand how single cells and cell ensembles behave under perturbation, which is essential for both basic research and clinical application. Various approaches have been developed to study cell-to-cell difference at the single-cell level, among which electrochemiluminescence (ECL) single-cell analysis has emerged in recent years. This Minireview focuses on the advances in ECL single cell analysis. After a brief introduction to single cell analysis and ECL, this overview mainly covers the ECL mechanisms and systems involved in ECL cytosensors, as well as the applications in ECL single cell analysis, which are classified into two main categories, namely intensity- and imaging-based methods. Finally, the outlooks and challenges in this field are discussed.
细胞异质性在任何细胞群体中都存在。了解单细胞和细胞群体在扰动下的行为是基础，这对基础研究和临床应用都至关重要。已经开发了各种方法来在单细胞水平上研究细胞间的差异，其中电化学发光 (ECL) 单细胞分析在近年来崭露头角。本综述重点介绍了 ECL 单细胞分析的进展。在简要介绍单细胞分析和 ECL 之后，本综述主要涵盖了 ECL 细胞传感器涉及的 ECL 机制和系统，以及 ECL 单细胞分析中的应用，分为基于强度和成像两种主要方法。最后，讨论了该领域的展望和挑战。
