Hoy Carlton F O, Kushiro Keiichiro, Yamaoka Yutaro, Ryo Akihide, Takai Madoka
Department of Bioengineering, School of Engineering, The University of Tokyo, Tokyo, Japan.
Department of Microbiology, School of Medicine, Yokohama City University, Yokohama, Japan.
Sens Biosensing Res. 2019 Nov;26:100304. doi: 10.1016/j.sbsr.2019.100304. Epub 2019 Oct 14.
On-site multiplex biosensors for innate immunity antibodies are ideal tools for monitoring health status of individuals against various diseases. This study introduces a novel antibody immunoassay testing platform incorporating microfiber-based arrays of antigens to capture specific antibodies. The fabrication and setup of the device revolved around electrospun polystyrene (ESPS) microfibers that act as three-dimensional membrane filters, capable of rapid and multifold analyte capture. In particular, the ESPS microfibers were patterned through localized oxygen plasma to create hydrophilic zones that facilitate fluid flows and immobilizations of antigens. The bulk of this robust antibody immunoassay platform could be installed into a compact syringe-driven cassette device, which could perform multiplex antibody immunoassay for antibodies specifically against Middle East respiratory syndrome coronavirus (MERS-CoV) with rapid preparation amounting to a total of 5 min, as well as high sensitivity and specificity for the MERS-CoV down to 200 μg/mL.
用于检测先天性免疫抗体的现场多重生物传感器是监测个体针对各种疾病的健康状况的理想工具。本研究引入了一种新型抗体免疫分析测试平台，该平台包含基于微纤维的抗原阵列以捕获特定抗体。该设备的制造和设置围绕着静电纺丝聚苯乙烯（ESPS）微纤维展开，这些微纤维充当三维膜过滤器，能够快速且多次捕获分析物。特别是，通过局部氧等离子体对ESPS微纤维进行图案化处理，以创建促进流体流动和抗原固定的亲水区。这种强大的抗体免疫分析平台的主体部分可以安装到一个紧凑的注射器驱动盒式装置中，该装置可以针对中东呼吸综合征冠状病毒（MERS-CoV）特异性抗体进行多重抗体免疫分析，快速制备时间总计为5分钟，并且对MERS-CoV的检测灵敏度和特异性高，低至200μg/mL。
