Rutkowski Rico, Daeschlein Georg, von Woedtke Thomas, Smeets Ralf, Gosau Martin, Metelmann Hans-Robert
Department of Oral and Maxillofacial Surgery, University Medicine Hamburg-Eppendorf, 20251 Hamburg, Germany.
Department of Dermatology, University Medicine Greifswald, 17475 Greifswald, Germany.
Diagnostics (Basel). 2020 Apr 11;10(4):210. doi: 10.3390/diagnostics10040210.
Despite increasing knowledge gained based on multidisciplinary research, plasma medicine still raises various questions regarding specific effects as well as potential risks. With regard to significant statements about in vivo applicability that cannot be prognosticated exclusively based on in vitro data, there is still a deficit of clinical data. This study included a clinical follow-up of five probands who had participated five years previously in a study on the influence of cold atmospheric pressure plasma (CAP) on the wound healing of CO laser-induced skin lesions. The follow-up included a complex imaging diagnostic involving dermatoscopy, confocal laser scanning microscopy (CLSM) and hyperspectral imaging (HSI). Hyperspectral analysis showed no relevant microcirculatory differences between plasma-treated and non-plasma-treated areas. In summary of all the findings, no malignant changes, inflammatory reactions or pathological changes in cell architecture could be detected in the plasma-treated areas. These unique in vivo long-term data contribute to a further increase in knowledge about important safety aspects in regenerative plasma medicine. However, to confirm these findings and secure indication-specific dose recommendations, further clinical studies are required.
尽管基于多学科研究获得了越来越多的知识，但等离子体医学在特定效应以及潜在风险方面仍然存在各种问题。关于无法仅根据体外数据预测的体内适用性的重要陈述，临床数据仍然不足。本研究对五名受试者进行了临床随访，他们五年前参与了一项关于冷大气压等离子体（CAP）对CO激光诱导的皮肤损伤伤口愈合影响的研究。随访包括一项复杂的成像诊断，涉及皮肤镜检查、共聚焦激光扫描显微镜（CLSM）和高光谱成像（HSI）。高光谱分析显示，等离子体处理区域和非等离子体处理区域之间没有相关的微循环差异。综合所有发现，在等离子体处理区域未检测到恶性变化、炎症反应或细胞结构的病理变化。这些独特的体内长期数据有助于进一步增加对再生等离子体医学重要安全方面的认识。然而，为了证实这些发现并确定特定适应症的剂量建议，还需要进一步的临床研究。
