Yang Qing, Chen Qiuju, Zhu Yuandi, Li Tianzhong
Laboratory of Fruit Cell and Molecular Breeding, China Agricultural University, Beijing 100193, China.
Funct Plant Biol. 2018 Apr;45(5):528-541. doi: 10.1071/FP17288.
As a classic plant-specific transcription factor family - the Dof domain proteins - are involved in a variety of biological processes in organisms ranging from unicellular Chlamydomonas to higher plants. However, there are limited reports of MdDof (Malus domestica Borkh. DNA-binding One Zinc Finger) domain proteins in fruit trees, especially in apple. In this study we identified 54 putative Dof transcription factors in the apple genome. We analysed the gene structures, protein motifs, and chromosome locations of each of the MdDof genes. Next, we characterised all 54 MdDofs their expression patterns under different abiotic and biotic stress conditions. It was found that MdDof6,26 not only played an important role in the biotic/abiotic stress but may also be involved in many molecular functions. Further, both in flower development and pollen tube growth it was found that the relative expression of MdDof24 increased rapidly, also with gene ontology analysis it was indicated that MdDof24 was involved in the chemical reaction and flower development pathways. Taken together, our results provide useful clues as to the function of MdDof genes in apple and serve as a reference for studies of Dof zinc finger genes in other plants.
作为一个经典的植物特异性转录因子家族，Dof结构域蛋白参与了从单细胞衣藻到高等植物等多种生物体的各种生物学过程。然而，关于果树中MdDof（苹果DNA结合单锌指蛋白）结构域蛋白的报道有限，尤其是在苹果中。在本研究中，我们在苹果基因组中鉴定出54个假定的Dof转录因子。我们分析了每个MdDof基因的基因结构、蛋白质基序和染色体定位。接下来，我们对所有54个MdDof在不同非生物和生物胁迫条件下的表达模式进行了表征。结果发现，MdDof6、26不仅在生物/非生物胁迫中起重要作用，还可能参与许多分子功能。此外，在花发育和花粉管生长过程中均发现MdDof24的相对表达迅速增加，基因本体分析也表明MdDof24参与化学反应和花发育途径。综上所述，我们的结果为苹果中MdDof基因的功能提供了有用线索，并为其他植物中Dof锌指基因的研究提供了参考。
