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鉴定和描述 基因家族及其在非生物胁迫-干旱、热和光胁迫中的作用。

Identification and Characterization of the Gene Family in and Its Role in Abiotic Stress-Drought, Heat and Light Stress.

机构信息

College of Computer and Information Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China.

College of Forestry, Fujian Agriculture and Forestry University, Fuzhou 350002, China.

出版信息

Int J Mol Sci. 2024 Oct 17;25(20):11147. doi: 10.3390/ijms252011147.

Abstract

is a second-class endangered and protected species unique to China, and it holds significant ecological and economic value. DNA binding one zinc finger (Dof) transcription factors are plant-specific regulators. Numerous studies have demonstrated that genes are involved in plant growth, development and responses to abiotic stress. In this study, we identified and analyzed 34 gene members at the whole-genome level. The results indicated that the 34 genes were unevenly distributed across 12 chromosomes. We utilized the genes from and to construct a phylogenetic tree and categorized these genes into eight subgroups. In the collinearity analysis, there were 16 homologous gene pairs between and and nine homologous gene pairs between and . We conducted a -acting element analysis and found that -acting elements involved in light response were the most abundant in genes. Through SSR site prediction, we analyzed that the evolution level of genes is low. Additionally, we assessed the expression profiles of eight genes under high temperature, drought, and light stress using qRT-PCR. In particular, and are significantly upregulated under the three stresses. This study provides foundational information for genes and offers new insights for further research on the mechanism of Dof transcription factors responding to stress, as well as the adaptation of to environmental changes.

摘要

是中国特有的二级濒危和保护物种,具有重要的生态和经济价值。DNA 结合锌指(Dof)转录因子是植物特有的调控因子。大量研究表明, 基因参与植物的生长、发育和对非生物胁迫的响应。在本研究中,我们在全基因组水平上鉴定和分析了 34 个 基因成员。结果表明,这 34 个基因在 12 条染色体上不均匀分布。我们利用 和 中的 基因构建了一个系统发育树,并将这些基因分为八个亚组。在共线性分析中, 和 之间有 16 对同源基因, 和 之间有 9 对同源基因。我们进行了 - 作用元件分析,发现 基因中与光反应相关的 - 作用元件最为丰富。通过 SSR 位点预测,我们分析了 基因的进化水平较低。此外,我们通过 qRT-PCR 评估了 8 个 基因在高温、干旱和光胁迫下的表达谱。特别是在这三种胁迫下, 和 显著上调。本研究为 基因提供了基础信息,为进一步研究 Dof 转录因子响应胁迫的机制以及 对环境变化的适应提供了新的见解。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3258/11508201/5858bbf69ef6/ijms-25-11147-g001.jpg

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