Department of Radiology, Sun Yat-sen Memorial Hospital, Sun Yat-sen University, Guangzhou, 510120, China.
MOE Key Laboratory of Bioinorganic and Synthetic Chemistry, School of Chemistry, Sun Yat-sen University, Guangzhou, 510275, China.
Angew Chem Int Ed Engl. 2020 Jun 2;59(23):8786-8798. doi: 10.1002/anie.201916474. Epub 2020 Mar 25.
Cell-free enzymatic catalysis (CFEC) is an emerging biotechnology that enable the biological transformations in complex natural networks to be imitated. This biomimetic approach allows industrial products such as biofuels and biochemical to be manufactured in a green manner. Nevertheless, the main challenge in CFEC is the poor stability, which restricts the effectiveness and lifetime of enzymes in sophisticated applications. Immobilization of the enzymes within solid carriers is considered an efficient strategy for addressing these obstacles. Specifically, putting an "armor-like" porous metal-organic framework (MOF) exoskeleton tightly around the enzymes not only shields the enzymes against external stimulus, but also allows the selective transport of guests through the accessible porous network. Herein we present the concept of this biotechnology of MOF-entrapped enzymes and its cutting-edge applications.
无细胞酶催化 (CFEC) 是一种新兴的生物技术，可模拟复杂天然网络中的生物转化。这种仿生方法可以以绿色方式制造生物燃料和生物化学等工业产品。然而，CFEC 的主要挑战是酶的稳定性差，这限制了酶在复杂应用中的有效性和寿命。将酶固定在固体载体中被认为是解决这些障碍的有效策略。具体来说，将“盔甲状”多孔金属-有机骨架 (MOF) 外壳紧紧地包裹在酶周围，不仅可以保护酶免受外部刺激，还可以允许通过可访问的多孔网络选择性地输送客体。本文介绍了 MOF 包埋酶的生物技术及其前沿应用。
