Department of Thoracic Surgery, The First Affiliated Hospital of Nanchang University, Nanchang, Jiangxi, 330006, China.
School of Public Health, Nanchang University, Nanchang, Jiangxi 330006, China.
Biosci Rep. 2020 Apr 30;40(4). doi: 10.1042/BSR20200065.
The tripartite motif (TRIM) family is a family of proteins with highly conserved domains. Previous researches have suggested that the members of TRIM family proteins played a crucial role in cancer development and progression. Our study explored the relationship between TRIM35 and non-small cell lung cancer (NSCLC). The study showed that the expression of TRIM35 was increased in NSCLC samples, and patients with high expression of TRIM35 had a poor clinical prognosis. Overexpression of TRIM35 in NSCLC cell line H460 promoted cell proliferation, migration, and invasion, knockdown of TRIM35 produced an opposite result in A549 and H1299 cell lines. In vivo study further confirmed that overexpression of TRIM35 promoted tumor formation. The RNA-seq analysis suggested that TRIM35 might promote lung cancer proliferation, migration, and invasion by regulating cancer-associated functions and signaling pathways. Hence, we identified TRIM35 played a significant role in tumoral growth and was a potential diagnosis and prognosis target for lung cancer.
三结构域蛋白家族（TRIM）是一类具有高度保守结构域的蛋白家族。先前的研究表明，TRIM 家族蛋白成员在癌症的发生和发展中起着至关重要的作用。本研究探讨了 TRIM35 与非小细胞肺癌（NSCLC）之间的关系。研究表明，TRIM35 在 NSCLC 样本中的表达增加，并且高表达 TRIM35 的患者临床预后较差。在 NSCLC 细胞系 H460 中过表达 TRIM35 促进了细胞增殖、迁移和侵袭，而在 A549 和 H1299 细胞系中敲低 TRIM35 则产生了相反的结果。体内研究进一步证实，过表达 TRIM35 促进了肿瘤的形成。RNA-seq 分析表明，TRIM35 可能通过调节癌症相关功能和信号通路促进肺癌的增殖、迁移和侵袭。因此，我们确定 TRIM35 在肿瘤生长中起着重要作用，是肺癌潜在的诊断和预后靶点。
