Jiangsu Provincial Key Lab for Organic Solid Waste Utilization, Jiangsu Collaborative Innovation Center for Solid Organic Wastes, Educational Ministry Engineering Center of Resource-saving fertilizers, Nanjing Agricultural University, Nanjing, 210095, China.
BMC Plant Biol. 2020 Apr 15;20(1):166. doi: 10.1186/s12870-020-02365-9.
Rhizosphere microbiome is dynamic and influenced by environment factors surrounded including pathogen invasion. We studied the effects of Ralstonia solanacearum pathogen abundance on rhizosphere microbiome and metabolome by using high throughput sequencing and GC-MS technology.
There is significant difference between two rhizosphere bacterial communities of higher or lower pathogen abundance, and this difference of microbiomes was significant even ignoring the existence of pathogen. Higher pathogen abundance decreased the alpha diversity of rhizosphere bacterial community as well as connections in co-occurrence networks. Several bacterial groups such as Bacillus and Chitinophaga were negatively related to the pathogen abundance. The GC-MS analysis revealed significantly different metabolomes in two groups of rhizosphere soils, i.e., the rhizosphere soil of lower harbored more sugars such as fructose, sucrose and melibiose than that in high pathogen abundance.
The dissimilar metabolomes in two rhizosphere soils likely explained the difference of bacterial communities with Mantel test. Bacillus and Chitinophaga as well as sugar compounds negatively correlated with high abundance of pathogen indicated their potential biocontrol ability.
根际微生物组是动态的，受周围环境因素的影响，包括病原体的入侵。我们通过高通量测序和 GC-MS 技术研究了青枯菌病原体丰度对根际微生物组和代谢组的影响。
高丰度和低丰度病原体的根际细菌群落之间存在显著差异，即使忽略病原体的存在，这种微生物组的差异也是显著的。较高的病原体丰度降低了根际细菌群落的 alpha 多样性以及共生网络中的连接性。一些细菌群，如芽孢杆菌和噬几丁质菌，与病原体丰度呈负相关。GC-MS 分析显示，两组根际土壤的代谢组存在显著差异，即低病原体丰度组的根际土壤中果糖、蔗糖和棉子糖等糖含量高于高病原体丰度组。
通过 Mantel 检验，两种根际土壤中不同的代谢组可能解释了细菌群落的差异。芽孢杆菌和噬几丁质菌以及与高病原体丰度呈负相关的糖化合物表明它们具有潜在的生物防治能力。
