Fang Wei, Zhu Yiyang, Liang Chenfei, Shao Shuai, Chen Junhui, Qing Hua, Xu Qiufang
State Key Laboratory of Subtropical Silviculture, Zhejiang A&F University, Hangzhou, China.
School of Environmental and Resource Sciences, Zhejiang A&F University, Hangzhou, China.
Front Microbiol. 2024 Nov 11;15:1448675. doi: 10.3389/fmicb.2024.1448675. eCollection 2024.
Fusarium-induced root rot of () is a typical soil-borne disease that has severely damaged the industry in China. Understanding the interaction among soil microbial communities, soil characteristics, and pathogenic bacteria is very important for the ecological prevention and control of root rot.
We used Miseq Illumina high-throughput sequencing technology to study the microbial community in the rhizosphere soil of healthy and diseased C. cathayensis, quantified the abundance of bacteria, fungi, and pathogenic fungi, and combined these with soil chemistry and enzyme activity indicators to analyze the characteristics of healthy and diseased rhizosphere soils.
We found that the pH, soil organic carbon(SOC), available nitrogen (AN), available phosphorus (AP), available potassium (AK),N-acetyl--D-glucosaminidase (NAG) β-glucosidase (BG), fungal gene copy number, bacterial community diversity and network complexity of the diseased soil were significantly lower ( < 0.05), while copies number levels increased ( < 0.05). Additionally, the study found that healthy soils were enriched with beneficial bacteria such as (0.08%), (0.29%), (0.08%), and (0.09%), as well as potential pathogen-suppressing fungi such as (0.13%), (0.03%), and (0.37%), were found to be associated with the growth and development of .
In summary, this research comprehensively reveals the differences in environmental and biological factors between healthy and diseased soils, as well as their correlations. It provides a theoretical basis for optimal soil environmental regulation and the construction of healthy microbial communities. This foundation facilitates the development of multifaceted strategies for the prevention and control of root rot.
镰刀菌引起的（）根腐病是一种典型的土传病害，已对我国的（）产业造成严重破坏。了解土壤微生物群落、土壤特性和病原菌之间的相互作用对于（）根腐病的生态防治非常重要。
我们使用Miseq Illumina高通量测序技术研究健康和患病（）的根际土壤中的微生物群落，量化细菌、真菌和致病真菌的丰度，并将这些与土壤化学和酶活性指标相结合，以分析健康和患病根际土壤的特征。
我们发现，患病土壤的pH值、土壤有机碳（SOC）、有效氮（AN）、有效磷（AP）、有效钾（AK）、N-乙酰-β-D-氨基葡萄糖苷酶（NAG）、β-葡萄糖苷酶（BG）、真菌基因拷贝数、细菌群落多样性和网络复杂性显著降低（<0.05），而（）拷贝数水平增加（<0.05）。此外，研究发现健康土壤富含有益细菌，如（0.08%）、（0.29%）、（0.08%）和（0.09%），以及潜在的抑制病原菌的真菌，如（0.13%）、（0.03%）和（0.37%），这些都与（）的生长发育有关。
综上所述，本研究全面揭示了健康和患病土壤之间环境和生物因素的差异及其相关性。它为优化土壤环境调控和构建健康微生物群落提供了理论依据。这一基础有助于制定多方面的（）根腐病防治策略。
