Antonysamy Johnson Marimuthu Alias, Shibila Thangaiah, Amutha Santhanam, Menezes Irwin R A, da Costa José G M, Sampaio Nadghia F L, Coutinho Henrique D M
Centre for Plant Biotechnology, Department of Botany, St. Xavier's College (Autonomous), Palayamkottai, Tamil Nadu- 627 002, India.
National Centre for Nanoscience and Nanotechnology, University of Madras, Chennai, Tamil Nadu 600025, India.
Pharmaceuticals (Basel). 2020 Apr 13;13(4):66. doi: 10.3390/ph13040066.
The present study was aimed to synthesize silver nanoparticles (AgNPs) from the aqueous extracts of (L.) J. Sm. and the synthesized AgNPs were examined for their biopotentials. The extracts were added to 1 mM AgNO solution with different ratios viz., 0.5: 9.5, 1:9, 1.5: 8.5 and 2: 8 ratios for the reduction of Ag ions. After reduction, the AgNPs of were analyzed spectroscopically for further confirmation. The synthesized AgNPs of were characterized by pH, ultra violet-visible spectroscopy (UV-Vis), Fourier transform-infra red spectroscopy (FT-IR), scanning electron microscopy-energy dispersive X-ray analysis (SEM-EDAX) and X-Ray diffraction (XRD). The time taken for the complete reduction of Silver (Ag) in solution to nanoparticle was 10 min. The aqueous extracts mediated silver nanoparticles showed a broad peak with distinct absorption at around 400-420 nm and confirmed the silver nanoparticle formation. FT-IR results also confirmed the existence of organic materials in the silver nanoparticles of . The EDX spectra of AgNPs of revealed the occurrence of a strong Ag peak. The synthesis of AgNPs of was confirmed with the existence of a peak at 46.228°. The toxic potential of AgNPs of showed varied percentage mortality with the LC values of 134.68 μL/ 50 mL and 76.5 μL/50 mL, respectively. The anti-inflammatory and anti-diabetic activities of aqueous and AgNPs of were statistically significant at < 0.05 level. Conclusion: The results demonstrated the toxicity, anti-diabetic and anti-inflammatory potential of the studied AgNPs The synthesized nanoparticles of could be tested as an alternative to anticancer, anti-diabetic and anti-inflammatory drugs.
本研究旨在从（植物名称未完整给出）的水提取物中合成银纳米颗粒（AgNPs），并对合成的AgNPs的生物活性进行检测。将该植物提取物以不同比例（即0.5:9.5、1:9、1.5:8.5和2:8）添加到1 mM硝酸银（AgNO₃）溶液中以还原银离子。还原后，通过光谱分析对该植物的AgNPs进行进一步确认。通过pH值、紫外可见光谱（UV-Vis）、傅里叶变换红外光谱（FT-IR）、扫描电子显微镜-能量色散X射线分析（SEM-EDAX）和X射线衍射（XRD）对合成的该植物AgNPs进行表征。溶液中银（Ag）完全还原为纳米颗粒所需时间为10分钟。该植物水提取物介导的银纳米颗粒在400 - 420 nm左右呈现出一个宽峰且有明显吸收，证实了银纳米颗粒的形成。FT-IR结果也证实了该植物银纳米颗粒中存在有机物质。该植物AgNPs的EDX光谱显示出强烈的Ag峰。在46.228°处出现的一个峰证实了该植物AgNPs的合成。该植物AgNPs的毒性潜力显示出不同的死亡率百分比，LC值分别为134.68 μL/50 mL和76.5 μL/50 mL。该植物水提取物和AgNPs的抗炎和抗糖尿病活性在P < 0.05水平具有统计学意义。结论：结果证明了所研究的AgNPs的毒性、抗糖尿病和抗炎潜力。合成的该植物纳米颗粒可作为抗癌、抗糖尿病和抗炎药物的替代品进行测试。
