Department of Infectious Diseases, College of Veterinary Medicine, University of Georgia, Athens, GA 30602, USA.
Viruses. 2020 Apr 14;12(4):439. doi: 10.3390/v12040439.
Human norovirus (HuNoV) is a principal cause of acute gastroenteritis worldwide, particularly in developing countries. Its global prevalence is underscored by more serious morbidity and some mortality in the young (<5 years) and the elderly. To date, there are no licensed vaccines or approved therapeutics for HuNoV, mostly because there are limited cell culture systems and small animal models available. Recently described cell culture systems are not ideal substrates for HuNoV vaccine development because they are not clonal or only support a single strain. In this study, we show Vero cell-based replication of two pandemic GII.4 HuNoV strains and one GII.3 strain and confirm exosome-mediated HuNoV infection in Vero cells. Lastly, we show that trypsin addition to virus cultures or disruption of Vero cell host genes can modestly increase HuNoV replication. These data provide support for Vero cells as a cell culture model for HuNoV.
人类诺如病毒（HuNoV）是全世界急性胃肠炎的主要病因，尤其在发展中国家。其在全球的流行率突显了在幼儿（<5 岁）和老年人中发病率更高和一些死亡率。迄今为止，尚无针对 HuNoV 的许可疫苗或批准的治疗方法，主要是因为可用的细胞培养系统和小动物模型有限。最近描述的细胞培养系统不是 HuNoV 疫苗开发的理想基质，因为它们不是克隆的，或者只支持单一株。在这项研究中，我们展示了基于 Vero 细胞的两种大流行 GII.4 HuNoV 株和一种 GII.3 株的复制，并证实了外泌体介导的 Vero 细胞 HuNoV 感染。最后，我们表明，向病毒培养物中添加胰蛋白酶或破坏 Vero 细胞宿主基因可以适度增加 HuNoV 的复制。这些数据为 Vero 细胞作为 HuNoV 的细胞培养模型提供了支持。
