Department of Mechanical Engineering, University of Louisville, Louisville, KY, 40292, USA.
Anal Bioanal Chem. 2020 Jun;412(16):3813-3833. doi: 10.1007/s00216-020-02590-z. Epub 2020 Apr 15.
Isomotive dielectrophoresis (isoDEP) is a unique electric field such that the gradient of the field-squared ([Formula: see text]) is constant, resulting a uniform dielectrophoretic force. The current status of isoDEP is presented in this review, and we will highlight the progress that has been achieved over the past 60 years in various avenues of isoDEP since H.A. Pohl initially described its premise. This article will discuss its applications and describe the various configurations of generating an isomotive force. Since H.A. Pohl introduced the theory of isoDEP, numerous authors have implemented isoDEP as a tool for the manipulation, sorting, separation, and characterization of polarizable particles without the need for biochemical labels or other bioengineered tagging. The growing field of microfluidics and electrokinetics has renewed interest in isoDEP, particularly for analytical characterization or separation of particles. Recent work has demonstrated that isoDEP can address some unmet needs for biomedical applications including single-cell analysis; moreover, advances in throughput as well as combining characterization and separation simultaneously will add significant value to isoDEP.
等速电泳（isoDEP）是一种独特的电场，其场平方的梯度（[公式：见正文]）保持恒定，从而产生均匀的电泳力。本文综述了等速电泳的现状，重点介绍了自 H.A. Pohl 最初描述其原理以来，过去 60 年来在等速电泳各个方面取得的进展。本文将讨论其应用，并描述产生等速力的各种配置。自 H.A. Pohl 提出等速电泳理论以来，许多作者已经将其用作无生物化学标记或其他生物工程标记的可极化粒子的操纵、分类、分离和特征化的工具。微流控和电动学领域的不断发展，重新引起了人们对等速电泳的兴趣，特别是对于粒子的分析特性或分离。最近的工作表明，等速电泳可以解决一些生物医学应用中未满足的需求，包括单细胞分析；此外，在提高通量的同时，同时进行特性分析和分离，将为等速电泳带来巨大的价值。
