Institute of Food Chemistry, Westfälische Wilhelms-Universität Münster, 48149 Münster, Germany.
J Agric Food Chem. 2020 May 13;68(19):5455-5461. doi: 10.1021/acs.jafc.0c00576. Epub 2020 Apr 29.
The type A trichothecene mycotoxins T-2 and HT-2 toxin are fungal secondary metabolites produced by fungi, which contaminate food and feed worldwide. Especially as a result of the high toxicity of T-2 toxin and their occurrence together with glucosylated forms in cereal crops, these mycotoxins are of human health concern. Particularly, it is unknown whether and how these modified mycotoxins are metabolized in the gastrointestinal tract and, thus, contribute to the overall toxicity. Therefore, the comparative intestinal metabolism of T-2 and HT-2 toxin glucosides in α and β configuration was investigated using the pig cecum model, which mimics the human intestinal metabolism. Regardless of its configuration, the C-3 glycosidic bond was hydrolyzed within 10-20 min, releasing T-2 and HT-2 toxin, which were further metabolized to HT-2 toxin and T-2 triol, respectively. We conclude that T-2 and HT-2 toxin should be evaluated together with their modified forms for risk assessment.
A型单端孢霉烯族毒素 T-2 和 HT-2 毒素是真菌产生的真菌次级代谢物，在全世界污染食物和饲料。特别是由于 T-2 毒素的高毒性及其与葡糖苷化形式一起出现在谷物作物中，这些霉菌毒素引起了人们对人类健康的关注。特别是，尚不清楚这些修饰的霉菌毒素是否以及如何在胃肠道中代谢，从而对整体毒性产生影响。因此，使用模拟人类肠道代谢的猪盲肠模型研究了 α 和 β 构型的 T-2 和 HT-2 毒素葡糖苷的比较肠道代谢。无论其构型如何，C-3 糖苷键都在 10-20 分钟内被水解，释放出 T-2 和 HT-2 毒素，然后进一步代谢为 HT-2 毒素和 T-2 三醇。我们得出结论，应将 T-2 和 HT-2 毒素与其修饰形式一起进行风险评估。
