网膜素-1改善游离脂肪酸诱导的人脐静脉内皮细胞增殖、迁移及炎症状态损伤。
Omentin-1 Ameliorated Free Fatty Acid-Induced Impairment in Proliferation, Migration, and Inflammatory States of HUVECs.
作者信息
Chen Yubin, Liu Fen, Han Fei, Lv Lizhi, Tang Can-E, Luo Fanyan
机构信息
Department of Cardiac Surgery, Xiangya Hospital, Central South University, Changsha 410008, Hunan, China.
The Institute of Medical Science Research, Xiangya Hospital, Central South University, Changsha 410008, Hunan, China.
出版信息
Cardiol Res Pract. 2020 Mar 27;2020:3054379. doi: 10.1155/2020/3054379. eCollection 2020.
OBJECTIVES
Endothelial cell injury is a critical pathological change during the development of atherosclerosis. Here, we explored the effect of omentin-1 on free fatty acid- (FFA-) induced endothelial cell injury.
METHODS
An FFA-induced endothelial cell injury model was established to investigate the role of omentin-1 in this process. Cell proliferation was analyzed with the Cell Counting Kit assay and flow cytometry. Scratch and transwell assays were used to evaluate cell migration. Factors secreted by endothelial cells after injury were detected by western blotting, reverse-transcription quantitative polymerase chain reaction, and cellular fluorescence assay.
RESULTS
Omentin-1 rescued the FFA-induced impaired proliferation and migration capabilities of human umbilical vein endothelial cells (HUVECs). It decreased the number of THP-1 cells attached to HUVECs in response to injury and inhibited the FFA-induced proinflammatory state of HUVECs.
CONCLUSION
Omentin-1 could partly ameliorate FFA-induced endothelial cell injury.
目的
内皮细胞损伤是动脉粥样硬化发展过程中的关键病理变化。在此，我们探讨了网膜素-1对游离脂肪酸（FFA）诱导的内皮细胞损伤的影响。
方法
建立FFA诱导的内皮细胞损伤模型，以研究网膜素-1在此过程中的作用。用细胞计数试剂盒检测法和流式细胞术分析细胞增殖。划痕试验和transwell试验用于评估细胞迁移。通过蛋白质印迹法、逆转录定量聚合酶链反应和细胞荧光测定法检测损伤后内皮细胞分泌的因子。
结果
网膜素-1挽救了FFA诱导的人脐静脉内皮细胞（HUVECs）增殖和迁移能力受损的情况。它减少了损伤后附着于HUVECs的THP-1细胞数量，并抑制了FFA诱导的HUVECs促炎状态。
结论
网膜素-1可部分改善FFA诱导的内皮细胞损伤。
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