Gaspardo Alba, Zannoni Augusta, Turroni Silvia, Barone Monica, Sabetti Maria Chiara, Zanoni Renato Giulio, Forni Monica, Brigidi Patrizia, Pietra Marco
Department of Veterinary Medical Sciences, University of Bologna, Ozzano Dell'Emilia, Italy.
Health Sciences and Technologies - Interdepartmental Center for Industrial Research (CIRI-SDV), University of Bologna, Bologna, Italy.
Front Vet Sci. 2020 Apr 2;7:146. doi: 10.3389/fvets.2020.00146. eCollection 2020.
The increasing incidence of gastrointestinal tract pathologies in dogs and the worrisome topic of antibiotic resistance have raised the need to look for new therapeutic frontiers. Of these, the use of probiotics represents a potential therapeutic alternative. () is a species of isolated from kefir. Previous studies have demonstrated that its administration in mice downregulates the expression of proinflammatory mediators and increases anti-inflammatory molecules in the gut immune system. It also regulates intestinal homeostasis, incrementing immunoglobulin A (IgA) secretion. Since has never been studied as a single probiotic in dogs, the aim of this study was to evaluate the safety of in dogs, and its effect on IgA secretion and on intestinal microbiota composition. Ten healthy dogs without a history of gastrointestinal diseases were included. The dogs received at a dose of 10 live microorganisms orally, once daily for 30 days. The fecal samples were tested before administration, in the middle, at the end, and 30 days after discontinuation. The IgA secretion concentration and the microbiota composition were evaluated on the fecal samples. The results in this study suggested that did not influence the concentration of IgA, nor significant changes of the intestinal microbiota were observed during and after the treatment. Therefore, additional studies are needed to investigate if a higher daily dosage of can influence the intestinal homeostasis of dogs.
犬胃肠道疾病发病率的不断上升以及令人担忧的抗生素耐药性问题，引发了寻找新治疗前沿的需求。其中，益生菌的使用代表了一种潜在的治疗选择。（）是一种从开菲尔中分离出来的菌种。先前的研究表明，在小鼠中施用该菌种可下调促炎介质的表达，并增加肠道免疫系统中的抗炎分子。它还调节肠道稳态，增加免疫球蛋白A（IgA）的分泌。由于从未将（）作为单一益生菌在犬中进行研究，本研究的目的是评估其在犬中的安全性，以及对IgA分泌和肠道微生物群组成的影响。纳入了10只无胃肠道疾病病史的健康犬。这些犬每天口服一次剂量为10个活微生物的（），持续30天。在给药前、给药中期、给药结束时以及停药后30天对粪便样本进行检测。对粪便样本评估IgA分泌浓度和微生物群组成。本研究结果表明，（）不影响IgA浓度，在治疗期间和治疗后也未观察到肠道微生物群的显著变化。因此，需要进一步研究以调查更高剂量的（）是否会影响犬的肠道稳态。
