Wang Dunfang, Shi KaiFeng, Wang Yanli, Zou Dixin, Guo Shanshan, Li Tao, Xu Hangyu, Ma Xuran, Liu JiaXing, Song HongXin, Yang Weipeng, Li Yu
Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China.
Wang Jing Hospital, China Academy of Chinese Medical Sciences, Beijing 100102, China.
Int J Genomics. 2020 Mar 26;2020:4238757. doi: 10.1155/2020/4238757. eCollection 2020.
In this study, we explored the pharmacological mechanisms of Huangqin Tang (HQT; a traditional Chinese medicine formula) in ulcerative colitis (UC) and provided evidence for potential roles HQT plays by gene expression profiling. The UC rat model was made via a compound method (trinitrobenzene sulfonic acid plus ethanol). After a ten-day treatment, microarray analysis was performed from the colon segment of the rats. Biological functions and specific signaling pathways were enriched based on differentially expressed genes (DEG), and corresponding gene networks were constructed via Ingenuity Pathway Analysis (IPA). Through the network, we screened the potential "candidate targets," such as ITGB1, FN1, CASP3, and ITGA5 and FABP1, ABCB1, FABP2, and SLC51B. These potential candidate targets were functionally related to immune responses, inflammation, and metabolism. Moreover, HQT significantly decreased serum levels of proinflammatory factors nitrogen monoxide (NO), proinflammatory cytokines interleukin- (IL-) 17, and prostaglandin E2 (PGE2). The degree of HE staining of colonic tissue was severe in the model group but reduced significantly in the HQT group. HQT exhibited protective effects against colon damage by inhibiting the inflammatory response.
在本研究中，我们探究了黄芩汤（HQT；一种中药配方）在溃疡性结肠炎（UC）中的药理机制，并通过基因表达谱分析为HQT发挥的潜在作用提供证据。UC大鼠模型通过复合方法（三硝基苯磺酸加乙醇）构建。经过十天的治疗后，对大鼠的结肠段进行微阵列分析。基于差异表达基因（DEG）富集生物学功能和特定信号通路，并通过Ingenuity通路分析（IPA）构建相应的基因网络。通过该网络，我们筛选出潜在的“候选靶点”，如整合素β1（ITGB1）、纤连蛋白1（FN1）、半胱天冬酶3（CASP3）以及整合素α5（ITGA5）和脂肪酸结合蛋白1（FABP1）、ATP结合盒转运蛋白B1（ABCB1）、脂肪酸结合蛋白2（FABP2）和溶质载体家族51成员B（SLC51B）。这些潜在的候选靶点在功能上与免疫反应、炎症和代谢相关。此外，HQT显著降低了促炎因子一氧化氮（NO）、促炎细胞因子白细胞介素 - 17（IL - 17）和前列腺素E2（PGE2）的血清水平。模型组结肠组织的苏木精 - 伊红（HE）染色程度严重，但HQT组显著减轻。HQT通过抑制炎症反应对结肠损伤表现出保护作用。
